PMID- 10389978
OWN - NLM
STAT- MEDLINE
DCOM- 19990707
LR  - 20181113
IS  - 0007-0920 (Print)
IS  - 0007-0920 (Linking)
VI  - 80
IP  - 1-2
DP  - 1999 Apr
TI  - Genetic alterations in hepatocellular carcinomas: association between loss of
      chromosome 4q and p53 gene mutations.
PG  - 59-66
AB  - The major risk factors for hepatocellular carcinomas (HCC) in high incidence
      areas include infection with hepatitis B and C viruses (HBV, HCV) and exposure to
      aflatoxin. Genetic alterations in 24 liver resection specimens from Shanghai and 
      Qidong were studied. Hepatitis B virus was integrated in all patient samples, and
      a null phenotype for the GSTM1 enzyme was present in 63% of patients. Alteration 
      of p53 was present in 95% (23/24) of cases: mutations of the p53 gene in 12 HCC, 
      p53 overexpression in 13 and loss of heterozygosity (LOH) of chromosome 17p in
      17. All seven HCCs with a p53 mutation from Qidong and three of five from
      Shanghai had the aflatoxin-associated point mutation with a G to T transversion
      at codon 249, position 3. No HCC had microsatellite instability. LOH of
      chromosome 4q, 1p, 16q and 13q was present in 50%, 46%, 42% and 38%,
      respectively, and 4q was preferentially lost in HCCs containing a p53 mutation:
      LOH of 4q was present in 75% (9/12) of HCC with, but only 25% (3/12) of HCC
      without, a p53 gene mutation (P = 0.01). These data indicate a possible
      interaction between p53 gene mutation and 4q loss in the pathogenesis of HCC.
FAU - Rashid, A
AU  - Rashid A
AD  - Department of Pathology, The Johns Hopkins Oncology Center, The John Hopkins
      School of Medicine, Baltimore, MD 21205-2196, USA.
FAU - Wang, J S
AU  - Wang JS
FAU - Qian, G S
AU  - Qian GS
FAU - Lu, B X
AU  - Lu BX
FAU - Hamilton, S R
AU  - Hamilton SR
FAU - Groopman, J D
AU  - Groopman JD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Hepatitis B Antigens)
RN  - 0 (Hepatitis C Antigens)
RN  - 9007-49-2 (DNA)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.5.1.18 (glutathione S-transferase M1)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Carcinoma, Hepatocellular/*genetics/pathology/virology
MH  - China
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 4/*genetics
MH  - DNA/analysis
MH  - Epoxide Hydrolases/genetics
MH  - *Genes, p53/genetics
MH  - Glutathione Transferase/genetics
MH  - Hepatitis B/complications/diagnosis
MH  - Hepatitis B Antigens/isolation & purification
MH  - Hepatitis C Antigens/isolation & purification
MH  - Humans
MH  - Immunohistochemistry
MH  - Liver Neoplasms/*genetics/pathology/virology
MH  - *Loss of Heterozygosity
MH  - Microsatellite Repeats
MH  - Mutation
MH  - Polymerase Chain Reaction
PMC - PMC2362990
EDAT- 1999/07/02 00:00
MHDA- 1999/07/02 00:01
CRDT- 1999/07/02 00:00
PHST- 1999/07/02 00:00 [pubmed]
PHST- 1999/07/02 00:01 [medline]
PHST- 1999/07/02 00:00 [entrez]
AID - 10.1038/sj.bjc.6690321 [doi]
PST - ppublish
SO  - Br J Cancer. 1999 Apr;80(1-2):59-66. doi: 10.1038/sj.bjc.6690321.