PMID- 10388667
OWN - NLM
STAT- MEDLINE
DCOM- 19990727
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 259
IP  - 2
DP  - 1999 Jul 5
TI  - The entire nucleotide sequence of a TT virus isolate from the United States
      (TUS01): comparison with reported isolates and phylogenetic analysis.
PG  - 437-48
AB  - A nonenveloped and single-stranded DNA virus designated TT virus (TTV) has been
      reported from Japan in association with hepatitis of unknown etiology. Very
      recently, the prototype TTV isolate (TA278) of genotype 1 is proven to have a
      circular genome with 3852 nucleotides. A TTV isolate (TUS01) was recovered from a
      blood donor in the United States, and its entire circular nucleotide sequence of 
      3818 nucleotides was determined. It possessed two open reading frames coding for 
      761 and 156 amino acids, respectively. TUS01 shared 60.5% of the nucleotide
      sequence with the TA278 isolate from Japan that was longer by 35 nt. The sequence
      of the noncoding region of 1203 nt was conserved with a similarity of 83.4%.
      Sequence preservation was much lower for the two open reading frames; nucleotide 
      and amino acid sequences were 54.8 and 37.0% similar, respectively, for one and
      55.5 and 38.8% similar for the other. By comparison of a partial sequence of 222 
      nucleotides among 239 TTV isolates available from various countries, at least 11 
      genotypes with sequence divergence of >30% were recognized. TUS01 was deduced to 
      be of genotype 11, which has not been reported before. Conserved sequences in the
      noncoding region could be used as primers for sensitively detecting TTV DNA by
      polymerase chain reaction. Divergent sequences in coding regions would be useful 
      as primers for distinguishing various TTV genotypes.
CI  - Copyright 1999 Academic Press.
FAU - Okamoto, H
AU  - Okamoto H
AD  - Immunology Division and Division of Molecular Virology, Jichi Medical School,
      Tochigi-Ken, 329-0498, Japan.
FAU - Nishizawa, T
AU  - Nishizawa T
FAU - Ukita, M
AU  - Ukita M
FAU - Takahashi, M
AU  - Takahashi M
FAU - Fukuda, M
AU  - Fukuda M
FAU - Iizuka, H
AU  - Iizuka H
FAU - Miyakawa, Y
AU  - Miyakawa Y
FAU - Mayumi, M
AU  - Mayumi M
LA  - eng
SI  - GENBANK/AB017610
SI  - GENBANK/AB017613
SI  - GENBANK/AB017772
SI  - GENBANK/AB017773
SI  - GENBANK/AB017774
SI  - GENBANK/AB017775
SI  - GENBANK/AB017776
SI  - GENBANK/AB017777
SI  - GENBANK/AB017778
SI  - GENBANK/AB017779
SI  - GENBANK/AB017780
SI  - GENBANK/AB017781
SI  - GENBANK/AB017782
SI  - GENBANK/AB017783
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blood Donors
MH  - DNA Virus Infections/*virology
MH  - DNA Viruses/chemistry/*genetics/isolation & purification
MH  - DNA, Viral/*genetics
MH  - Genotype
MH  - Hepatitis Viruses/chemistry/*genetics/isolation & purification
MH  - Hepatitis, Viral, Human/*virology
MH  - Humans
MH  - Japan
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Open Reading Frames
MH  - *Phylogeny
MH  - Sequence Analysis, DNA
MH  - United States
EDAT- 1999/07/02 00:00
MHDA- 1999/07/02 00:01
CRDT- 1999/07/02 00:00
PHST- 1999/07/02 00:00 [pubmed]
PHST- 1999/07/02 00:01 [medline]
PHST- 1999/07/02 00:00 [entrez]
AID - 10.1006/viro.1999.9769 [doi]
AID - S0042-6822(99)99769-6 [pii]
PST - ppublish
SO  - Virology. 1999 Jul 5;259(2):437-48. doi: 10.1006/viro.1999.9769.