PMID- 10388521
OWN - NLM
STAT- MEDLINE
DCOM- 19990825
LR  - 20131121
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 250
IP  - 1
DP  - 1999 Jul 10
TI  - Colocalization within the nucleolus of two highly related IFN-induced human
      nuclear phosphoproteins with nucleolin.
PG  - 62-74
AB  - We have previously reported the identification of two interferon (IFN)-induced
      cDNAs which code for two proteins, named 41 and 75, which have homology to a
      number of proteins involved in regulating gene expression. Here we establish that
      these cDNAs correspond to in vivo synthesized mRNAs. Expression of the 41 and 75 
      cDNAs, both in vitro and in vivo, generated proteins of 30 and 68 kDa,
      respectively. In a variety of mammalian cells, 41 and 75 were found to be located
      in the nucleus, with 41 being localized to the nucleolus, whereas 75, although it
      is mainly concentrated at the periphery of the nucleolus, is also found
      throughout the nucleoplasm. Treatment with interferon results in a translocation 
      of 41 to the periphery of the nucleolus and it is in this region that the two
      proteins colocalize. 41 and 75 were found to colocalize with nucleolin but not
      with B23 or fibrillarin, three nucleolar proteins involved in ribosome synthesis.
      This colocalization was not affected by low concentrations of actinomycin D. In
      view of this and since 41 and 75 have homology to proteins involved in regulating
      gene expression, we suggest that, in association with nucleolin, they play a role
      in ribosome biogenesis.
CI  - Copyright 1999 Academic Press.
FAU - Welsh, G I
AU  - Welsh GI
AD  - Unite de Virologie et d'Immunologie Cellulaire, Institut Pasteur, Paris cedex 15,
      75724, France.
FAU - Kadereit, S
AU  - Kadereit S
FAU - Coccia, E M
AU  - Coccia EM
FAU - Hovanessian, A G
AU  - Hovanessian AG
FAU - Meurs, E F
AU  - Meurs EF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interferon-alpha)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (fibrillarin)
RN  - 0 (nuclear phosphoprotein 41)
RN  - 0 (nuclear phosphoprotein 75)
RN  - 0 (nucleolin)
RN  - 117896-08-9 (nucleophosmin)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cell Nucleolus/*chemistry
MH  - Chromosomal Proteins, Non-Histone/analysis
MH  - DNA, Complementary
MH  - Dactinomycin/pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - Interferon-alpha/*pharmacology
MH  - Interferon-gamma/*pharmacology
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*analysis/genetics
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Phosphoproteins/*analysis/genetics
MH  - RNA, Messenger
MH  - RNA-Binding Proteins/*analysis
MH  - Recombinant Fusion Proteins/analysis/genetics
MH  - Ribonucleoproteins/analysis
MH  - Sequence Homology, Amino Acid
MH  - U937 Cells
EDAT- 1999/07/02 00:00
MHDA- 1999/07/02 00:01
CRDT- 1999/07/02 00:00
PHST- 1999/07/02 00:00 [pubmed]
PHST- 1999/07/02 00:01 [medline]
PHST- 1999/07/02 00:00 [entrez]
AID - 10.1006/excr.1999.4505 [doi]
AID - S0014-4827(99)94505-6 [pii]
PST - ppublish
SO  - Exp Cell Res. 1999 Jul 10;250(1):62-74. doi: 10.1006/excr.1999.4505.