PMID- 10386950
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 1
DP  - 1999 Jul
TI  - Molecular cloning of testican-2: defining a novel calcium-binding proteoglycan
      family expressed in brain.
PG  - 12-20
AB  - We have screened a human cDNA library using an expressed sequence tag related to 
      the BM-40/secreted protein, acidic and rich in cysteine (SPARC)/osteonectin
      family of proteins and isolated a novel cDNA. It encodes a protein precursor of
      424 amino acids that consists of a signal peptide, a follistatin-like domain, a
      Ca2+-binding domain, a thyroglobulin-like domain, and a C-terminal region with
      two putative glycosaminoglycan attachment sites. The protein is homologous to
      testican-1 and was termed testican-2. Testican-1 is a proteoglycan originally
      isolated from human seminal plasma that is also expressed in brain. Northern blot
      hybridization of testican-2 showed a 6.1-kb mRNA expressed mainly in CNS but also
      found in lung and testis. A widespread expression in multiple neuronal cell types
      in olfactory bulb, cerebral cortex, thalamus, hippocampus, cerebellum, and
      medulla was detected by in situ hybridization. A recombinant fragment consisting 
      of the Ca2+-binding EF-hand domain and the thyroglobulin-like domain of
      testican-2 showed a reversible Ca2+-dependent conformational change in circular
      dichroism studies. Testican-1 and -2 form a novel Ca2+-binding proteoglycan
      family built of modular domains with the potential to participate in diverse
      steps of neurogenesis.
FAU - Vannahme, C
AU  - Vannahme C
AD  - Institute for Biochemistry II, Medical Faculty, University of Cologne, Germany.
FAU - Schubel, S
AU  - Schubel S
FAU - Herud, M
AU  - Herud M
FAU - Gosling, S
AU  - Gosling S
FAU - Hulsmann, H
AU  - Hulsmann H
FAU - Paulsson, M
AU  - Paulsson M
FAU - Hartmann, U
AU  - Hartmann U
FAU - Maurer, P
AU  - Maurer P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (DNA, Complementary)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 0 (SPOCK1 protein, human)
RN  - 0 (SPOCK2 protein, human)
RN  - 0 (Spock1 protein, mouse)
RN  - 0 (Spock2 protein, mouse)
RN  - 0 (Testicular Hormones)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Brain Chemistry
MH  - Calcium/*metabolism
MH  - *Cloning, Molecular
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Fetus
MH  - Humans
MH  - In Situ Hybridization
MH  - Lung/chemistry
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteoglycans/chemistry/*genetics/metabolism
MH  - RNA, Messenger/analysis
MH  - Testicular Hormones/genetics
MH  - Testis/chemistry
MH  - Tissue Distribution
EDAT- 1999/07/01 00:00
MHDA- 1999/07/01 00:01
CRDT- 1999/07/01 00:00
PHST- 1999/07/01 00:00 [pubmed]
PHST- 1999/07/01 00:01 [medline]
PHST- 1999/07/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0730012.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Jul;73(1):12-20. doi: 10.1046/j.1471-4159.1999.0730012.x.