PMID- 10386602
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 452
IP  - 3
DP  - 1999 Jun 11
TI  - Anisoosmotic regulation of the Nopp140 mRNA in H4IIE rat hepatoma cells and
      primary hepatocytes.
PG  - 259-62
AB  - Using the differential display polymerase chain reaction osmosensitive regulation
      of mRNA levels of the nucleolar phosphoprotein of 140 kDa (Nopp140) was found in 
      H4IIE rat hepatoma cells. These levels were downregulated after hypoosmotic
      exposure in H4IIE cells and primary rat hepatocytes. Hyperosmotic incubation
      increased Nopp140 mRNA levels in H4IIE cells but not in hepatocytes. Inhibition
      of p38MAPK or MAP kinase kinase upstream of Erk-1 and Erk-2 decreased Nopp140
      mRNA levels but did not prevent their osmosensitivity. Because Nopp140 is
      involved in the regulation of transcriptional activity it could play a role in
      the osmosignalling pathway towards gene expression in H4IIE cells and
      hepatocytes.
FAU - Warskulat, U
AU  - Warskulat U
AD  - Medizinische Universitatsklinik der Heinrich-Heine-Universitat Dusseldorf, Klinik
      fur Gastroenterologie, Hepatologie und Infektiologie, Germany.
FAU - Hammermann, R
AU  - Hammermann R
FAU - Haussinger, D
AU  - Haussinger D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Hypertonic Solutions)
RN  - 0 (Imidazoles)
RN  - 0 (NOLC1 protein, human)
RN  - 0 (Nolc1 protein, rat)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Pyridines)
RN  - 0 (RNA, Messenger)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - OU13V1EYWQ (SB 203580)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/metabolism
MH  - Cell Nucleolus/metabolism
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - *Gene Expression Regulation
MH  - Gene Expression Regulation, Neoplastic
MH  - Hypertonic Solutions
MH  - Imidazoles/pharmacology
MH  - Kinetics
MH  - Liver/*metabolism
MH  - Liver Neoplasms, Experimental/genetics/*metabolism
MH  - Male
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - *Mitogen-Activated Protein Kinases
MH  - Nuclear Proteins/*genetics
MH  - Phosphoproteins/*genetics
MH  - Protein Kinase Inhibitors
MH  - Pyridines/pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - *Transcription, Genetic/drug effects
MH  - Water-Electrolyte Balance
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 1999/07/01 00:00
MHDA- 1999/07/01 00:01
CRDT- 1999/07/01 00:00
PHST- 1999/07/01 00:00 [pubmed]
PHST- 1999/07/01 00:01 [medline]
PHST- 1999/07/01 00:00 [entrez]
AID - S0014-5793(99)00643-2 [pii]
AID - 10.1016/s0014-5793(99)00643-2 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jun 11;452(3):259-62. doi: 10.1016/s0014-5793(99)00643-2.