PMID- 10384036
OWN - NLM
STAT- MEDLINE
DCOM- 19990720
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 7
DP  - 1999 Jul
TI  - Genetic and physical maps of the mouse rd3 locus; exclusion of the ortholog of
      USH2A.
PG  - 657-61
AB  - The rd3 retinal degeneration gene was previously mapped 10 +/- 2.5 cM distal to
      Akp1 on mouse Chromosome (Chr) 1 (Chang et al., 1993), a region that may be
      homologous to the locus of the human USH2A gene, which carries mutations
      responsible for Usher IIa retinal degeneration/hearing loss syndrome. An
      intercross from an Rb(11, 13)4Bnr(rd3/rd3) x C57BL/6J mating was set up, 428 F2
      meioses were analyzed, and the rd3 gene was placed between the markers
      D1MIT292/D1MIT209 and D1MIT510, a distance of 1.40 +/- 0.57 cM. These flanking
      markers and the mouse ortholog of USH2A (Mush2a) were mapped in the T31 mouse
      radiation hybrid (RH) panel, with the result that D1MIT292/D1MIT209 and D1MIT510 
      were 7.9 cR3000 apart ( approximately 800 kb), and Mush2a was > 30 cR3000
      proximal to the pair, excluding it from the rd3 locus. A contig spanning the rd3 
      locus and consisting of 2 YACs and one BAC was generated, and Mush2a was absent
      from it, confirming its exclusion from the locus. Comparison of adjacent marker
      pairs in the Whitehead genetic map and our genetic map showed some discrepancies 
      in order of markers and genetic distances. Comparison of our genetic map and the 
      RH map showed some highly skewed relationships between genetic and physical
      distances.
FAU - Danciger, J S
AU  - Danciger JS
AD  - Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California
      90095-7008, USA.
FAU - Danciger, M
AU  - Danciger M
FAU - Nusinowitz, S
AU  - Nusinowitz S
FAU - Rickabaugh, T
AU  - Rickabaugh T
FAU - Farber, D B
AU  - Farber DB
LA  - eng
GR  - EY00331/EY/NEI NIH HHS/United States
GR  - EY08285/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (Ush2a protein, mouse)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - DNA Primers
MH  - Extracellular Matrix Proteins/*genetics
MH  - Genetic Markers
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Retinal Degeneration/*genetics
EDAT- 1999/06/29 00:00
MHDA- 1999/06/29 00:01
CRDT- 1999/06/29 00:00
PHST- 1999/06/29 00:00 [pubmed]
PHST- 1999/06/29 00:01 [medline]
PHST- 1999/06/29 00:00 [entrez]
AID - MG99-632 [pii]
AID - 10.1007/s003359901067 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Jul;10(7):657-61. doi: 10.1007/s003359901067.