PMID- 10383826
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20071115
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 13
IP  - 6
DP  - 1999 Jun
TI  - Rapid phosphorylation of Elk-1 transcription factor and activation of MAP kinase 
      signal transduction pathways in response to visual stimulation.
PG  - 405-14
AB  - The AP-1 transcription factor, which is composed of various combinations of Fos
      and Jun proteins, is believed to be a key participant in molecular processes that
      guide activity-dependent changes in gene expression. In this study, we
      investigated the activity of different MAP kinases that have been implicated in
      AP-1 activation. We examined the activities of ERK, JNK/SAPK, and p38 MAPK along 
      with their nuclear targets (Elk-1 and c-Jun) in rat visual cortex after light
      stimulation. The transcription factor Elk-1 (a possible regulator of c-fos
      expression) was found to be transiently modified by phosphorylation when visual
      stimulation was applied after a period of dark rearing. In vitro kinase assay
      with Elk-1 as substrate showed that light stimulation activated MAPK/ERK in
      visual cortex but not frontal cortex. Furthermore, ERK activation was temporally 
      matched to onset of Elk-1 phosphorylation. The activity of JNK1 (c-Jun N-terminal
      kinase 1) was elevated at 2-6 h after visual exposure and was also temporally
      correlated to increase of endogenous P-c-Jun levels and its appearance within the
      AP-1 DNA-binding complex. The activities of p38 MAP kinases did not change
      significantly. These results demonstrate the differential engagement of MAPK
      signaling pathways following sensory stimulation and their relative effects upon 
      AP-1 expression in the intact brain.
CI  - Copyright 1999 Academic Press.
FAU - Kaminska, B
AU  - Kaminska B
AD  - Nencki Institute of Experimental Biology, Warsaw, Poland.
FAU - Kaczmarek, L
AU  - Kaczmarek L
FAU - Zangenehpour, S
AU  - Zangenehpour S
FAU - Chaudhuri, A
AU  - Chaudhuri A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Elk1 protein, rat)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Transcription Factors)
RN  - 0 (ets-Domain Protein Elk-1)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - *DNA-Binding Proteins
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Light
MH  - Male
MH  - Phosphorylation
MH  - Photic Stimulation
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Signal Transduction/*physiology
MH  - Transcription Factors/metabolism
MH  - Visual Cortex/metabolism/physiology
MH  - ets-Domain Protein Elk-1
EDAT- 1999/06/29 00:00
MHDA- 1999/06/29 00:01
CRDT- 1999/06/29 00:00
PHST- 1999/06/29 00:00 [pubmed]
PHST- 1999/06/29 00:01 [medline]
PHST- 1999/06/29 00:00 [entrez]
AID - 10.1006/mcne.1999.0757 [doi]
AID - S1044-7431(99)90757-1 [pii]
PST - ppublish
SO  - Mol Cell Neurosci. 1999 Jun;13(6):405-14. doi: 10.1006/mcne.1999.0757.