PMID- 10383739
OWN - NLM
STAT- MEDLINE
DCOM- 19990713
LR  - 20181201
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 112
IP  - 6
DP  - 1999 Jun
TI  - Serum xylosyltransferase: a new biochemical marker of the sclerotic process in
      systemic sclerosis.
PG  - 919-24
AB  - UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase (EC2.4.2.26) is 
      the initial enzyme in the biosynthesis of chondroitin sulfate and dermatan
      sulfate proteoglycans in fibroblasts and chondrocytes. Secretion of
      xylosyltransferase into the extracellular space was determined in cultured human 
      dermal fibroblasts. A more than 6-fold accumulation of xylosyltransferase
      activity in cell culture supernatant was observed (day 1, 0.6 microU per 106
      cells; day 9, 4.1 microU per 106 cells); however, intracellular
      xylosyltransferase activity remained at a constant level (0.4 microU per 106
      cells). Exposure of human chondrocytes to colchicine led to a 3-fold decreased
      level of xylosyltransferase and chondroitin-6-sulfate concentration in cell
      culture. Specific xylosyltransferase activity and chondroitin-6-sulfate
      concentration decreased in a concentration-dependent manner and in parallel in
      culture medium and accumulated 5-fold in cell lysates indicating that
      xylosyltransferase is secreted simultaneously into the extracellular space with
      chondroitin sulfate proteoglycans. Xylosyltransferase activities were determined 
      in serum samples of 30 patients with systemic sclerosis. Xylosyltransferase
      activities in female (mean value 1.28 mU per liter, 90% range 1.10-1.55 mU per
      liter) and male patients (mean 1.39 mU per liter, 90% range 1.16-1. 57 mU per
      liter) with systemic sclerosis were significantly increased in comparison with
      blood donors of a corresponding age. Furthermore, xylosyltransferase activity was
      correlated with the clinical classification of systemic sclerosis. Female
      patients with diffuse cutaneous systemic sclerosis showed higher serum
      xylosyltransferase activities than patients with limited systemic sclerosis.
      These results confirm that the increase of proteoglycan biosynthesis in sclerotic
      processes of scleroderma is closely related to an elevated xylosyltransferase
      activity in blood and demonstrate the validity of xylosyltransferase as an
      additional diagnostic marker for determination of sclerotic activity in systemic 
      sclerosis.
FAU - Gotting, C
AU  - Gotting C
AD  - Institut fur Laboratoriums-und Transfusionsmedizin, Herz-und Diabeteszentrum,
      Nodrhein-Westfalen, Universitatsklinik der Ruhr-Universitat Bochum, Bad
      Oeynhausen, Germany.
FAU - Sollberg, S
AU  - Sollberg S
FAU - Kuhn, J
AU  - Kuhn J
FAU - Weilke, C
AU  - Weilke C
FAU - Huerkamp, C
AU  - Huerkamp C
FAU - Brinkmann, T
AU  - Brinkmann T
FAU - Krieg, T
AU  - Krieg T
FAU - Kleesiek, K
AU  - Kleesiek K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Biomarkers)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - EC 2.4.2.- (Pentosyltransferases)
RN  - EC 2.4.2.26 (UDP xylose-protein xylosyltransferase)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/blood
MH  - Cartilage/cytology/drug effects/metabolism
MH  - Cells, Cultured
MH  - Chondroitin Sulfates/metabolism
MH  - Colchicine/pharmacology
MH  - Extracellular Space/metabolism
MH  - Female
MH  - Fibroblasts/enzymology
MH  - Humans
MH  - Longitudinal Studies
MH  - Male
MH  - Middle Aged
MH  - Pentosyltransferases/*blood
MH  - Scleroderma, Systemic/blood/enzymology/physiopathology
MH  - Skin/cytology/enzymology
EDAT- 1999/06/26 00:00
MHDA- 1999/06/26 00:01
CRDT- 1999/06/26 00:00
PHST- 1999/06/26 00:00 [pubmed]
PHST- 1999/06/26 00:01 [medline]
PHST- 1999/06/26 00:00 [entrez]
AID - S0022-202X(15)40508-1 [pii]
AID - 10.1046/j.1523-1747.1999.00590.x [doi]
PST - ppublish
SO  - J Invest Dermatol. 1999 Jun;112(6):919-24. doi: 10.1046/j.1523-1747.1999.00590.x.