PMID- 10381817
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 5
IP  - 7
DP  - 1999 Jul
TI  - Outer dense fibre proteins from human sperm tail: molecular cloning and
      expression analyses of two cDNA transcripts encoding proteins of approximately 70
      kDa.
PG  - 627-35
AB  - The outer dense fibres (ODF) are a main cytoskeletal structure of the sperm tail.
      Despite their importance in the morphology and function of the sperm tail, their 
      constituents are poorly described. Here we investigate the protein composition of
      human outer dense fibres. Our results suggest that human ODF consist of about 10 
      major and of at least 15 minor proteins, where all major proteins are ODF1, ODF2 
      or ODF2-related proteins. From a human testis cDNA library, we isolated two
      slightly different cDNAs encoding ODF2 proteins of approximately 70 kDa. Human
      ODF2 cDNAs and their encoded proteins are very similar to those isolated from rat
      and mouse pointing to a high evolutionary pressure residing on these proteins.
      Transcription of ODF2 is restricted to testis tissue and more specifically to
      round spermatids as was demonstrated by a non-radioactive in-situ hybridization. 
      ODF2 proteins were detected in the sperm tail. Their distribution along the
      length of the sperm tail shows that the ODF normally extend to about half the
      principal piece of the sperm tail. The former result opens the possibility for a 
      screening regarding the distribution of sperm tail proteins related to motility
      disorders.
FAU - Petersen, C
AU  - Petersen C
AD  - III Zoologisches Institut-Entwicklungsbiologie, Universitat Gottingen,
      Humboldtallee 34A, 37073 Gottingen, Germany.
FAU - Fuzesi, L
AU  - Fuzesi L
FAU - Hoyer-Fender, S
AU  - Hoyer-Fender S
LA  - eng
SI  - GENBANK/AF012549
SI  - GENBANK/AF053970
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (DNA, Complementary)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (ODF1 protein, human)
RN  - 0 (ODF2 protein, human)
RN  - 0 (Odf1 protein, mouse)
RN  - 0 (Odf1 protein, rat)
RN  - 0 (Odf2 protein, mouse)
RN  - 0 (Odf2 protein, rat)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Evolution, Molecular
MH  - *Heat-Shock Proteins
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Polymerase Chain Reaction
MH  - Proteins/chemistry/*genetics/isolation & purification
MH  - Rats
MH  - Recombinant Proteins/biosynthesis/chemistry/isolation & purification
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Sperm Tail/*metabolism
MH  - Testis/*metabolism
MH  - *Transcription, Genetic
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1093/molehr/5.7.627 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 1999 Jul;5(7):627-35. doi: 10.1093/molehr/5.7.627.