PMID- 10381623
OWN - NLM
STAT- MEDLINE
DCOM- 19990714
LR  - 20041117
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 6
IP  - 4
DP  - 1999 Apr
TI  - A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis induced by Nip3
      by forming a heterodimer through the C-terminal hydrophobic region.
PG  - 314-25
AB  - The adenovirus E1B19K protein inhibits apoptosis induced by E1A and other
      divergent signals. The cellular proteins that interact with E1B19K have been
      analyzed by isolating cDNA clones by the yeast two hybrid system. One of these
      clones encodes B5 which consists of 219 amino acid residues and contains the
      putative BH3 and transmembrane regions. B5 binds strongly to Nip3 and itself,
      weakly to E1B19K, but not to Bcl-2 and localizes in nuclear envelope, endoplasmic
      reticulum and mitochondria. B5 has sequence homology with Nip3 in the middle and 
      C-terminal regions, but not in the N-terminal region. Unlike other E1B19K binding
      BH3 proteins so far characterized, B5 does not induce apoptosis, but inhibits
      apoptosis induced by Nip3. However the deletion mutant B5Delta1-31 lacking the
      N-terminus does induce apoptosis, although weaker than does Nip3, suggesting that
      the N-terminal region is masking the apoptosis-inducing capacity of B5.
FAU - Ohi, N
AU  - Ohi N
AD  - Department of Biological Science and Technology, Science University of Tokyo,
      Noda, Japan.
FAU - Tokunaga, A
AU  - Tokunaga A
FAU - Tsunoda, H
AU  - Tsunoda H
FAU - Nakano, K
AU  - Nakano K
FAU - Haraguchi, K
AU  - Haraguchi K
FAU - Oda, K
AU  - Oda K
FAU - Motoyama, N
AU  - Motoyama N
FAU - Nakajima, T
AU  - Nakajima T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Adenovirus E1B Proteins)
RN  - 0 (BNIP3 protein, human)
RN  - 0 (BNIP3L protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (E1B19K-binding protein B5)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Adenovirus E1B Proteins/chemistry/genetics/*metabolism
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Base Sequence
MH  - Blotting, Western
MH  - COS Cells
MH  - Carcinoma, Squamous Cell
MH  - Carrier Proteins/analysis/*chemistry/*genetics
MH  - DNA, Complementary
MH  - Dimerization
MH  - Flow Cytometry
MH  - Gene Expression/physiology
MH  - Humans
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Protein Binding/physiology
MH  - Protein Structure, Tertiary
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/analysis
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/chemistry
MH  - Tumor Cells, Cultured/cytology/metabolism/virology
MH  - *Tumor Suppressor Proteins
MH  - *Viral Proteins
MH  - Yeasts/genetics
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1038/sj.cdd.4400493 [doi]
PST - ppublish
SO  - Cell Death Differ. 1999 Apr;6(4):314-25. doi: 10.1038/sj.cdd.4400493.