PMID- 10381567
OWN - NLM
STAT- MEDLINE
DCOM- 19990924
LR  - 20190910
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 83
IP  - 1-2
DP  - 1999 May
TI  - The PBX-regulating protein PREP1 is present in different PBX-complexed forms in
      mouse.
PG  - 53-64
AB  - Human PREP1, a novel homeodomain protein of the TALE super-family, forms a stable
      DNA-binding complex with PBX proteins in solution, a ternary complex with PBX and
      HOXB1 on DNA, and is able to act as a co-activator in the transcription of
      PBX-HOXB1 activated promoters (Berthelsen, J., Zappavigna, V., Ferretti, E.,
      Mavilio, F., Blasi, F. , 1998b. The novel homeoprotein Prep1 modulates Pbx-Hox
      protein cooperatity. EMBO J. 17, 1434-1445; Berthelsen, J., Zappavigna, V.,
      Mavilio, F., Blasi, F., 1998c. Prep1, a novel functional partner of Pbx proteins.
      EMBO J. 17, 1423-1433). Here we demonstrate the presence of DNA-binding PREP1-PBX
      complexes also in murine cells. In vivo, PREP1 is a predominant partner of PBX
      proteins in various murine tissues. However, the choice of PBX family member
      associated with PREP1 is largely tissue-type specific. We report the cloning and 
      expression domain of murine Prep1 gene. Murine PREP1 shares 100% identity with
      human PREP1 in the homeodomain and 95% similarity throughout the whole protein.
      In the adult mouse, PREP1 is expressed ubiquitously, with peaks in testis and
      thymus. We further demonstrate the presence of murine Prep1 mRNA and protein, and
      of different DNA-binding PREP1-PBX complexes, in mouse embryos from at least 9.5 
      days p.c. Moreover, we show that PREP1 is present in all embryonic tissues from
      at least 7.5-17.5 days p.c with a predominantly nuclear staining. PREP1 is able
      to super-activate the PBX-HOXB-1 autoregulated Hoxb-1 promoter, and we show that 
      all three proteins, PREP1, PBX and HOXB-1, are present together in the mouse
      rhombomere 4 domain in vivo, compatible with a role of PREP1 as a regulator of
      PBX and HOXB-1 proteins activity during development.
FAU - Ferretti, E
AU  - Ferretti E
AD  - Molecular Genetics Unit, Department of Biology and Biotechnology (DIBIT), H.S.
      San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy.
FAU - Schulz, H
AU  - Schulz H
FAU - Talarico, D
AU  - Talarico D
FAU - Blasi, F
AU  - Blasi F
FAU - Berthelsen, J
AU  - Berthelsen J
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (PKNOX1 protein, human)
RN  - 0 (Pknox1 protein, mouse)
RN  - 0 (Pre-B-Cell Leukemia Transcription Factor 1)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (pbx1 protein, human)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA-Binding Proteins/analysis/*metabolism
MH  - Embryo, Mammalian/anatomy & histology
MH  - Gene Expression Regulation, Developmental
MH  - Homeodomain Proteins/analysis/*metabolism
MH  - Humans
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Pre-B-Cell Leukemia Transcription Factor 1
MH  - Proto-Oncogene Proteins/analysis/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - Tissue Distribution
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S0925477399000313 [pii]
AID - 10.1016/s0925-4773(99)00031-3 [doi]
PST - ppublish
SO  - Mech Dev. 1999 May;83(1-2):53-64. doi: 10.1016/s0925-4773(99)00031-3.