PMID- 10381566
OWN - NLM
STAT- MEDLINE
DCOM- 19990924
LR  - 20190910
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 83
IP  - 1-2
DP  - 1999 May
TI  - N-myc-dependent repression of ndr1, a gene identified by direct subtraction of
      whole mouse embryo cDNAs between wild type and N-myc mutant.
PG  - 39-52
AB  - To identify genes regulated by N-myc, subtraction of whole embryo cDNA was
      carried out between wild type and N-myc-deficient mutant mice. Six cDNA clones
      were isolated as representing genes expressed higher in the mutant embryos and
      two as those expressed lower. One of them, Ndr1, coding for 43 kDa cytoplasmic
      protein was studied in detail. The Ndr1 gene was augmented 20-fold in the mutant 
      embryos at 10.5 days post coitus which is indicative of repression by N-myc. An
      inverse relationship actually existed between the expression of N-myc and Ndr1 in
      various developing tissues of the wild type embryos. In the early stage of
      differentiation of these tissues when N-myc expression was high Ndr1 expression
      was low or undetectable, and later when N-myc activity diminished Ndr1 expression
      was augmented concomitantly with the occurrence of terminal differentiation. To
      establish the direct link between N-myc activity and the Ndr1 regulation, the
      Ndr1 gene was cloned and analyzed. The Ndr1 promoter activity was down-regulated 
      by N-myc, and more strongly by the combination of N-myc and Max in the
      cotransfection assay. This repressive effect was mediated by the promoter region 
      within 52 base pairs from the transcription start site but direct binding of
      N-myc:Max to the promoter sequence was not demonstrated, which is analogous to
      the cases recently reported for transcriptional repression by c-myc. c-myc also
      repressed Ndr1 promoter activity similarly to N-myc. The effect of N-myc:Max was 
      sensitive to Trichostatin A, indicating involvement of histone deacetylase
      activity in repression of the Ndr1 promoter. The strategy we adopted in
      identifying target genes of a transcription factor should prove widely applicable
      when mutant animals are available.
FAU - Shimono, A
AU  - Shimono A
AD  - Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka,
      Suita, Osaka 565, Japan.
FAU - Okuda, T
AU  - Okuda T
FAU - Kondoh, H
AU  - Kondoh H
LA  - eng
SI  - GENBANK/AB004248
PT  - Journal Article
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (N-myc downstream-regulated gene 1 protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins
MH  - DNA, Complementary/*analysis
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Embryo, Mammalian/anatomy & histology/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Intracellular Signaling Peptides and Proteins
MH  - Luciferases/metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - *Nuclear Proteins
MH  - Plant Proteins/analysis/*metabolism
MH  - Proto-Oncogene Proteins c-myc/analysis/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Time Factors
MH  - *Transcription Factors
MH  - Up-Regulation
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S0925477399000258 [pii]
AID - 10.1016/s0925-4773(99)00025-8 [doi]
PST - ppublish
SO  - Mech Dev. 1999 May;83(1-2):39-52. doi: 10.1016/s0925-4773(99)00025-8.