PMID- 10381548
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20191210
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 70
IP  - 1
DP  - 1999 Jun 18
TI  - Characterization of multiple forms of the human glycine transporter type-2.
PG  - 101-15
AB  - The human glycine transporter type 2 (hGlyT2) was cloned from a spinal cord cDNA 
      library using PCR-based methodologies. The isolated sequence exhibits 89%
      homology with the previously isolated rat GlyT2 cDNA (Liu et al., J. Biol. Chem. 
      268 (1993) 22802-22808) at the nucleotide level, and 93% amino acid sequence
      identity. The greatest divergence between the human and rat sequences is found at
      the amino-terminus, where only 74% amino acid identity exists in residues 1-190. 
      Expression of the intact hGlyT2 transporter sequence in COS-7 cells resulted in a
      10-fold increase in high-affinity uptake relative to control cells transfected
      with vector alone. An artificially truncated form of the transporter, missing the
      NH2-terminal 153 amino acids, was also capable of mediating glycine uptake.
      However, an identified variant lacking the first 234 amino acids was
      non-functional. An hGlyT2 transporter containing a 14-residue deletion in the
      intracellular loop between transmembrane domains 6 and 7 was also identified and 
      expressed, but failed to mediate glycine uptake. Like rat GlyT2, the
      high-affinity uptake mediated by hGlyT2 was found to be insensitive to the GlyT1 
      inhibitor sarcosine.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Gallagher, M J
AU  - Gallagher MJ
AD  - Discovery Research, Gliatech, 23420 Commerce Park Rd., Cleveland, OH 44122, USA. 
      gallagherm@gliatech.com
FAU - Burgess, L H
AU  - Burgess LH
FAU - Brunden, K R
AU  - Brunden KR
LA  - eng
SI  - GENBANK/AF142501
GR  - R43-MH58037/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Amino Acid Transport Systems, Neutral)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycine Plasma Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SLC6A5 protein, human)
RN  - 0 (SLC6A9 protein, human)
RN  - 0 (Slc6a5 protein, rat)
RN  - 0 (Slc6a9 protein, rat)
RN  - Z711V88R5F (Sarcosine)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - *Amino Acid Transport Systems, Neutral
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport/drug effects
MH  - COS Cells
MH  - Carrier Proteins/antagonists & inhibitors/*classification/genetics/isolation &
      purification
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Glycine Plasma Membrane Transport Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/antagonists & inhibitors/*classification/genetics/isolation
      & purification
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms/antagonists & inhibitors/*classification/genetics/isolation &
      purification
MH  - Rats
MH  - Recombinant Fusion Proteins/antagonists & inhibitors/metabolism
MH  - Sarcosine/pharmacology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Spinal Cord/chemistry
MH  - Transfection
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S0169328X99001357 [pii]
AID - 10.1016/s0169-328x(99)00135-7 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Jun 18;70(1):101-15. doi:
      10.1016/s0169-328x(99)00135-7.