PMID- 10381362
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20100825
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 260
IP  - 1
DP  - 1999 Jun 24
TI  - Cloning and characterization of a novel orphan G-protein-coupled receptor
      localized to human chromosome 2p16.
PG  - 174-80
AB  - We report the identification and characterisation of a novel human orphan
      G-protein-coupled receptor (GPR) which maps to chromosome 2p16. We have
      determined the full-length coding sequence and genomic structure of a gene
      corresponding to the anonymous expressed sequenced tag, WI-31133. This gene
      encodes a novel protein that is 540 amino acids in length. Protein sequence
      analysis predicts the presence of seven transmembrane domains, a characteristic
      feature of GPRs. In situ hybridisation to human retina and Northern blot analysis
      of human retinal pigment epithelium (RPE) showed localisation of this transcript 
      to the RPE and cells surrounding retinal arterioles. In contrast, the transcript 
      was localised to the photoreceptor inner segments and the outer plexiform layer
      in mouse sections. Northern blot analysis demonstrated a 7 kb transcript highly
      expressed in the brain. No mutations were identified during a screen of patients 
      suffering from Doyne's honeycomb retinal dystrophy (DHRD), an inherited retinal
      degeneration which maps to chromosome 2p16.
CI  - Copyright 1999 Academic Press.
FAU - Tarttelin, E E
AU  - Tarttelin EE
AD  - Division of Biomedical Sciences, Imperial College School of Medicine, London, SW7
      2AZ, United Kingdom.
FAU - Kirschner, L S
AU  - Kirschner LS
FAU - Bellingham, J
AU  - Bellingham J
FAU - Baffi, J
AU  - Baffi J
FAU - Taymans, S E
AU  - Taymans SE
FAU - Gregory-Evans, K
AU  - Gregory-Evans K
FAU - Csaky, K
AU  - Csaky K
FAU - Stratakis, C A
AU  - Stratakis CA
FAU - Gregory-Evans, C Y
AU  - Gregory-Evans CY
LA  - eng
SI  - GENBANK/AF072693
SI  - GENBANK/AF101472
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (GPR75 protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 2
MH  - Cloning, Molecular
MH  - Expressed Sequence Tags
MH  - GTP-Binding Proteins/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic
MH  - *Receptors, Cell Surface/*chemistry/*genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Retinal Diseases/genetics
MH  - Retinal Vessels/metabolism
MH  - Tissue Distribution
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1006/bbrc.1999.0753 [doi]
AID - S0006-291X(99)90753-2 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 24;260(1):174-80. doi:
      10.1006/bbrc.1999.0753.