PMID- 10381349
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 260
IP  - 1
DP  - 1999 Jun 24
TI  - Impaired galactosylation of core 2 O-glycans in erythrocytes of
      beta1,4-galactosyltransferase knockout mice.
PG  - 94-8
AB  - O- and N-glycans included in erythrocyte membrane glycoproteins from
      beta1,4-galactosyltransferase I (GalT-I) knockout mice were analyzed to examine
      how this enzyme deficiency affects glycosylation of proteins in erythroid cells. 
      The results indicated that greater than 80% of core 2 O-glycans from GalT-I-/-
      mice are not galactosylated by beta1,4 linkage, resulting in the expression of
      Neu5Acalpha2 --> 3Galbeta1 --> 3(GlcNAcbeta1 --> 6)GalNAc, while core 2 O-glycans
      from GalT-I+/+ mice are fully galactosylated and occur as Neu5Acalpha2 -->
      3Galbeta1 --> 3(Neu5Acalpha2 --> 3Galbeta1 --> 4GlcNAcbeta1 --> 6)GalNAc. On the 
      other hand, beta1, 4-galactosylation of N-glycans of the mutant was approximately
      60% that of the wild type. Thus, it is suggested that GalT-I is predominantly
      responsible for beta1,4-galactosylation of the core 2 O-glycan branch in
      erythroid cells.
CI  - Copyright 1999 Academic Press.
FAU - Kotani, N
AU  - Kotani N
AD  - Department of Biochemistry, University of Tokyo, Tokyo, 108-8639, Japan.
FAU - Asano, M
AU  - Asano M
FAU - Iwakura, Y
AU  - Iwakura Y
FAU - Takasaki, S
AU  - Takasaki S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Membrane Proteins)
RN  - 0 (Polysaccharides)
RN  - EC 2.4.1.90 (N-Acetyllactosamine Synthase)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Animals
MH  - Carbohydrate Sequence
MH  - Erythrocytes/*metabolism
MH  - Galactose/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - N-Acetyllactosamine Synthase/genetics/*metabolism
MH  - Polysaccharides/*metabolism
MH  - Time Factors
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1006/bbrc.1999.0872 [doi]
AID - S0006-291X(99)90872-0 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 24;260(1):94-8. doi: 10.1006/bbrc.1999.0872.