PMID- 10380959
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 10
IP  - 7
DP  - 1999 May 14
TI  - GDNF, RET and GFRalpha-1-3 mRNA expression in the developing human spinal cord
      and ganglia.
PG  - 1433-9
AB  - The identification of endogenous neurotrophic factors and their receptors in
      human spinal cord is important not only to understand development, but also in
      the consideration of possible future therapies for neurodegenerative disorders
      and trauma. Using in situ hybridization, the expression of glial cell
      line-derived neurotrophic factor (GDNF), neurturin (NTN), persephin (PSP),
      GFRalpha-1, GFRalpha-2, GFRalpha-3 and RET mRNA in human fetal spinal cord was
      studied. Strong GDNF mRNA hybridization signal, presumably restricted to Clarke's
      nucleus, was detected in the thoracic spinal cord. mRNA encoding GFRalpha-1 was
      expressed in the entire spinal cord gray matter with particularly high expression
      in the ventral horn. GFRbeta-1 was also expressed more weakly in dorsal root
      ganglia. NTN and persephin mRNA were not detected in either the fetal spinal cord
      or the dorsal root ganglia. mRNA coding for GFRalpha-2, however, was found in
      most cells of the spinal cord gray matter. A strong expression of GFRalpha-3 mRNA
      was detected in dorsal root ganglia cells and Schwann cells. The transducing
      receptor RET was expressed strongly in motorneurons and dorsal root ganglion
      neurons. We conclude that basic features concerning the role of the GDNF family
      of ligands and their receptors revealed in rodents applies to humans.
FAU - Widenfalk, J
AU  - Widenfalk J
AD  - Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
FAU - Widmer, H R
AU  - Widmer HR
FAU - Spenger, C
AU  - Spenger C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Drosophila Proteins)
RN  - 0 (GDNF protein, human)
RN  - 0 (GFRA1 protein, human)
RN  - 0 (GFRA3 protein, human)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NRTN protein, human)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurturin)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (persephin)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Ret protein, Drosophila)
SB  - IM
MH  - *Drosophila Proteins
MH  - Embryonic and Fetal Development/physiology
MH  - Ganglia, Spinal/embryology/*metabolism
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glial Cell Line-Derived Neurotrophic Factor Receptors
MH  - Humans
MH  - In Situ Hybridization
MH  - Membrane Glycoproteins/*genetics
MH  - Nerve Growth Factors/genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - Neurturin
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-ret
MH  - RNA, Messenger/*biosynthesis
MH  - Receptor Protein-Tyrosine Kinases/genetics
MH  - Receptors, Cell Surface/genetics
MH  - *Receptors, Nerve Growth Factor
MH  - Spinal Cord/embryology/*metabolism
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1097/00001756-199905140-00009 [doi]
PST - ppublish
SO  - Neuroreport. 1999 May 14;10(7):1433-9. doi: 10.1097/00001756-199905140-00009.