PMID- 10380936
OWN - NLM
STAT- MEDLINE
DCOM- 19990901
LR  - 20191024
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 9
IP  - 2
DP  - 1999 Apr
TI  - Loss of heterozygosity at chromosome 9p21 (INK4-p14ARF locus): homozygous
      deletions and mutations in the p16 and p14ARF genes in sporadic primary
      melanomas.
PG  - 138-47
AB  - Loss of heterozygosity (LOH) was determined in 45 sporadic primary melanomas at
      six polymorphic microsatellite markers that flank the INK4a (p16-p14ARF) locus on
      chromosome 9p21. We also determined allelic loss at two markers on chromosome 9q 
      and two markers at the Rb locus on chromosome 13. Homozygous deletion of the p16 
      and p14ARF genes was determined by a fluorescent-based quantitative multiplex
      polymerase chain reaction method. LOH at one or more polymorphic microsatellite
      markers on locus 9p21 was found in 32 of the melanomas (71%). The highest
      proportion of LOH was found at markers D9S736 and D9S104, which are telomeric and
      centromeric to the INK4 locus, respectively. Five melanomas showed LOH at all the
      analysed markers located on chromosome 9p21. LOH at markers D9S942 and D9S974,
      which are located close to the p16 and p14ARF genes, was found in 39% and 46% of 
      melanomas, respectively. Analysis of the marker D9S257 on 9q22.1 showed LOH in 13
      melanomas (44% of the informative cases). A subset of melanomas with LOH at the
      INK4 locus also carried inactivating mutations within the p16 coding sequence.
      Four melanomas carried homozygous deletions at the p16-p14ARF locus. Our results 
      suggest, besides the involvement of the INK4 locus in sporadic melanomas, the
      possibility of the existence of additional tumour suppressor loci on chromosome
      9.
FAU - Kumar, R
AU  - Kumar R
AD  - Department of Biosciences, Karolinska Institute, Novum, Huddinge, Sweden.
      rajiv.kumar@cnt.ki.se
FAU - Smeds, J
AU  - Smeds J
FAU - Lundh Rozell, B
AU  - Lundh Rozell B
FAU - Hemminki, K
AU  - Hemminki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (Proteins)
RN  - 0 (Tumor Suppressor Protein p14ARF)
SB  - IM
MH  - Alleles
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 13
MH  - *Chromosomes, Human, Pair 9
MH  - Genes, p16/*genetics
MH  - Humans
MH  - *Loss of Heterozygosity
MH  - Melanoma/*genetics
MH  - Microsatellite Repeats
MH  - Models, Genetic
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proteins/*genetics
MH  - Sequence Analysis, DNA
MH  - Skin Neoplasms/*genetics
MH  - Tumor Suppressor Protein p14ARF
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1097/00008390-199904000-00005 [doi]
PST - ppublish
SO  - Melanoma Res. 1999 Apr;9(2):138-47. doi: 10.1097/00008390-199904000-00005.