PMID- 10380882
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20160108
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 24
DP  - 1999 Jun 17
TI  - MBP1: a novel mutant p53-specific protein partner with oncogenic properties.
PG  - 3608-16
AB  - Using a yeast two-hybrid screening strategy with a common tumour-derived p53
      mutant as bait, we identified several mutant p53-interacting partners including
      the known proteins wild-type (wt) p53, hUBC9 and GBP/PIAS1. In addition, a novel 
      protein partner was identified which we have termed MBP1, for Mutant p53-Binding 
      Protein 1. MBP1 is a new member of the emerging fibulin gene family, which
      currently comprises fibulin-1, fibulin-2 and S1-5. Expression of MBP1 mRNA is
      differentially regulated both temporally during development of the mouse embryo
      and in a tissue-specific manner within the adult. Specific interaction between
      MBP1 and mutant p53 was illustrated by both two-hybrid analysis in yeast and
      co-immunoprecipitation in mammalian cells. MBP1 displayed the following order of 
      binding specificity towards different p53 forms: H175 > G281 > H273 > or =
      W248>wt p53. Thus, MBP1 appears to bind preferentially to p53 mutants of the
      'structural' rather than 'contact' class, reflecting a potential bias towards
      those mutants having a significant alteration in conformation from that assumed
      by wt p53. We propose that MBP1 is the product of a candidate oncogene as rates
      of both neoplastic transformation and tumour cell growth were shown to be
      significantly enhanced when the protein is ectopically overexpressed.
      Furthermore, MBP1 may play a role in determining if a 'gain of function' effect
      is seen with certain p53 mutants.
FAU - Gallagher, W M
AU  - Gallagher WM
AD  - Oncology Department, Rhone-Poulenc Rorer, CRVA, Vitry-sur-Seine, France.
FAU - Argentini, M
AU  - Argentini M
FAU - Sierra, V
AU  - Sierra V
FAU - Bracco, L
AU  - Bracco L
FAU - Debussche, L
AU  - Debussche L
FAU - Conseiller, E
AU  - Conseiller E
LA  - eng
SI  - GENBANK/AF104223
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Efemp2 protein, mouse)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (fibulin)
RN  - 0 (fibulin-4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium-Binding Proteins/chemistry/genetics
MH  - Cell Division
MH  - *Cell Transformation, Neoplastic
MH  - Cloning, Molecular
MH  - Embryo, Mammalian/metabolism
MH  - Extracellular Matrix Proteins/chemistry/genetics/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Oncogene Proteins/chemistry/genetics/*metabolism
MH  - Organ Specificity
MH  - Protein Binding
MH  - Protein Conformation
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/chemistry/genetics/*metabolism
MH  - Yeasts/genetics/metabolism
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1038/sj.onc.1202937 [doi]
PST - ppublish
SO  - Oncogene. 1999 Jun 17;18(24):3608-16. doi: 10.1038/sj.onc.1202937.