PMID- 10380635
OWN - NLM
STAT- MEDLINE
DCOM- 19990721
LR  - 20190910
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 63
IP  - 5
DP  - 1999 May
TI  - Two isoforms of a human actin-related protein show nuclear localization and
      mutually selective expression between brain and other tissues.
PG  - 917-23
AB  - Actin-related proteins (Arps), which are divergent, but apparently homologues to 
      actin, are categorized into 10 classes. While Arps belonging to classes 1-3 were 
      found to be localized in the cytoplasm across eukaryotic phyla, other classes of 
      Arps were found mostly in invertebrates and suggested to contribute to structural
      modulation of chromatin. Here we report the identification and the
      characterization of two human isoforms of an Arp not belonging to classes 1-3,
      which we designated hArpN alpha and hArpN beta. Both proteins were expressed in
      HeLa cells and they were found localized within the nucleus. Most interestingly, 
      in different human tissues, hArpN alpha and beta were found to be expressed
      mutually exclusively, and the expression of hArpN alpha was absolutely restricted
      to the brain. These findings suggest that, in vertebrates, members of distantly
      related Arps might have tissue-specific functions in the nucleus, possibly
      through structural modulation of chromatin.
FAU - Harata, M
AU  - Harata M
AD  - Department of Molecular and Cell Biology, Graduate School of Agricultural
      Science, Tohoku University, Sendai, Japan. mharata@biochem.tohoku.ac.jp
FAU - Mochizuki, R
AU  - Mochizuki R
FAU - Mizuno, S
AU  - Mizuno S
LA  - eng
SI  - GENBANK/AB015906
SI  - GENBANK/AB015907
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Actins)
RN  - 0 (DNA Primers)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Actins/chemistry/genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - DNA Primers
MH  - Exons
MH  - HeLa Cells
MH  - Humans
MH  - Introns
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Protein Isoforms/chemistry/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - 10.1271/bbb.63.917 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 1999 May;63(5):917-23. doi: 10.1271/bbb.63.917.