PMID- 10379887
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20081121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 13
IP  - 6
DP  - 1999 Jun
TI  - Human placental TEF-5 transactivates the human chorionic somatomammotropin gene
      enhancer.
PG  - 879-89
AB  - Human chorionic somatomammotropin (hCS) gene expression in the placenta is
      controlled by an enhancer (CSEn) containing SV40-related GT-IIC and SphI/SphII
      enhansons. These enhancers are controlled by members of the transcription
      enhancer factor-1 (TEF-1) family. Recently TEF-5, whose mRNA is abundant in
      placenta, was shown to bind cooperatively to a unique, tandemly repeated element 
      in CSEn2, suggesting that TEF-5 regulates CSEn activity. However, expression of
      TEF-5 using a cDNA lacking the 5'-untranslated region and containing a modified
      translation initiation site was not accompanied by CSEn activation. Using nested,
      degenerate PCR primers corresponding to conserved TEF domains, several novel
      TEF-1-related cDNAs have been cloned from a human placental cDNA library. The
      open reading frame of one 3033-bp clone was identical to TEF-5 and contained 300-
      and 1423-bp 5'- and 3'-untranslated regions, respectively. The in vitro generated
      approximately 53-kDa TEF-5 polypeptide binds specifically to GT-IIC and
      SphI/SphII oligonucleotides. Overexpression of TEF-5 in BeWo cells using the
      intact 3033-bp cDNA transactivates the hCS and SV40 enhancers and artificial
      enhancers comprised of tandemly repeated GT-IIC enhansons, but not OCT enhansons.
      The data demonstrate that TEF-5 is a transactivator that is likely involved in
      the transactivation of CSEn enhancer function. Further, the data suggest that
      elements within the untranslated regions, initiation site, or both control TEF-5 
      expression in ways that influence its transactivation function.
FAU - Jiang, S W
AU  - Jiang SW
AD  - Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Wu, K
AU  - Wu K
FAU - Eberhardt, N L
AU  - Eberhardt NL
LA  - eng
SI  - GENBANK/AF142482
GR  - DK-41206/DK/NIDDK NIH HHS/United States
GR  - DK-51492/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (TEAD3 protein, human)
RN  - 0 (Transcription Factors)
RN  - 9035-54-5 (Placental Lactogen)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/genetics
MH  - Base Sequence
MH  - COS Cells/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics/*metabolism
MH  - *Enhancer Elements, Genetic
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/metabolism
MH  - Myocardium/metabolism
MH  - Placenta/metabolism
MH  - Placental Lactogen/*genetics/metabolism
MH  - Pregnancy
MH  - Transcription Factors/*genetics/*metabolism
EDAT- 1999/06/24 00:00
MHDA- 1999/06/24 00:01
CRDT- 1999/06/24 00:00
PHST- 1999/06/24 00:00 [pubmed]
PHST- 1999/06/24 00:01 [medline]
PHST- 1999/06/24 00:00 [entrez]
AID - 10.1210/mend.13.6.0288 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1999 Jun;13(6):879-89. doi: 10.1210/mend.13.6.0288.