PMID- 10377264
OWN - NLM
STAT- MEDLINE
DCOM- 19990903
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 341 ( Pt 1)
DP  - 1999 Jul 1
TI  - Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2
      and Grb7 in the c-Kit/stem cell factor receptor.
PG  - 211-6
AB  - In this paper we demonstrate the presence of two novel in vivo
      autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR):
      Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore
      demonstrate that the adapter protein Grb2 is a specific binding partner for both 
      phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein
      Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the
      association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7.
      Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the
      Ras/mitogen-activated protein kinase pathway.
FAU - Thommes, K
AU  - Thommes K
AD  - Ludwig Institute for Cancer Research, Biomedicum, Box 595, S-751 24 Uppsala,
      Sweden.
FAU - Lennartsson, J
AU  - Lennartsson J
FAU - Carlberg, M
AU  - Carlberg M
FAU - Ronnstrand, L
AU  - Ronnstrand L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (GRB2 protein, human)
RN  - 0 (GRB7 protein, human)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 149058-53-7 (GRB7 Adaptor Protein)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Cells, Cultured
MH  - Endothelium, Vascular/cytology
MH  - GRB2 Adaptor Protein
MH  - GRB7 Adaptor Protein
MH  - Humans
MH  - Phosphorylation
MH  - Protein Binding
MH  - Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-kit/genetics/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/genetics/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Stem Cell Factor
MH  - *Tyrosine
MH  - src Homology Domains
PMC - PMC1220349
EDAT- 1999/06/23 00:00
MHDA- 1999/06/23 00:01
CRDT- 1999/06/23 00:00
PHST- 1999/06/23 00:00 [pubmed]
PHST- 1999/06/23 00:01 [medline]
PHST- 1999/06/23 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Jul 1;341 ( Pt 1):211-6.