PMID- 10376525
OWN - NLM
STAT- MEDLINE
DCOM- 19990630
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 23
DP  - 1999 Jun 10
TI  - Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein
      kinase that signals apoptosis through its catalytic activity.
PG  - 3471-80
AB  - We have identified and characterized a new calcium/calmodulin (Ca2+/CaM)
      dependent protein kinase termed death-associated protein kinase 2 (DAPK2) that
      contains an N-terminal protein kinase domain followed by a conserved CaM-binding 
      domain with significant homologies to those of DAP kinase, a protein kinase
      involved in apoptosis. DAPK2 mRNA is expressed abundantly in heart, lung and
      skeletal muscle. The mapping results indicated that DAPK2 is located in the
      central region of mouse chromosome 9. In vitro kinase assay revealed that DAPK2
      is autophosphorylated and phosphorylates myosin light chain (MLC) as an exogenous
      substrate. DAPK2 binds directly to CaM and is activated in a Ca2+/CaM-dependent
      manner. A constitutively active DAPK2 mutant is generated by removal of the
      CaM-binding domain (deltaCaM). Treatment of agonists that elevate intracellular
      Ca2+-concentration led to the activation of DAPK2 and transfection studies
      revealed that DAPK2 is localized in the cytoplasm. Overexpression of DAPK2, but
      not the kinase negative mutant, significantly induced the morphological changes
      characteristic of apoptosis. These results indicate that DAPK2 is an additional
      member of DAP kinase family involved in apoptotic signaling.
FAU - Kawai, T
AU  - Kawai T
AD  - Department of Biochemistry, Hyogo College of Medicine, Nishinomiya, Japan.
FAU - Nomura, F
AU  - Nomura F
FAU - Hoshino, K
AU  - Hoshino K
FAU - Copeland, N G
AU  - Copeland NG
FAU - Gilbert, D J
AU  - Gilbert DJ
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Akira, S
AU  - Akira S
LA  - eng
SI  - GENBANK/AB018001
SI  - GENBANK/AB018002
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Calmodulin)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (DAPK2 protein, human)
RN  - EC 2.7.11.1 (Death-Associated Protein Kinases)
RN  - EC 2.7.11.1 (dapk2 protein, mouse)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Apoptosis Regulatory Proteins
MH  - COS Cells
MH  - Calcium/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*physiology
MH  - Calmodulin/metabolism
MH  - Chromosome Mapping
MH  - Death-Associated Protein Kinases
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Tissue Distribution
MH  - Transfection
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1038/sj.onc.1202701 [doi]
PST - ppublish
SO  - Oncogene. 1999 Jun 10;18(23):3471-80. doi: 10.1038/sj.onc.1202701.