PMID- 10376215
OWN - NLM
STAT- MEDLINE
DCOM- 19990913
LR  - 20211203
IS  - 1042-5179 (Print)
IS  - 1026-7913 (Linking)
VI  - 10
IP  - 2
DP  - 1999
TI  - A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like
      features of human Tmp21-II.
PG  - 121-6
AB  - Tmp21 (p23) is involved in biosynthetic transport from the endoplasmic reticulum 
      to the Golgi complex. We have recently characterized two cDNA-variants of human
      Tmp21, the Tmp21-isoforms-I and -II. Because of the lack of cDNA sequence data
      and protein expression data, it was not clear if Tmp21-II encodes a functional
      Tmp21-protein. Here we describe the cloning of the full length human Tmp21-II
      transcript. The putative open reading frame of Tmp21-II contains a reading frame 
      jump and a nonsense mutation in comparison to all other Tmp21-I (p23) members.
      Our data indicate that hum-Tmp21-II is transcribed, but not translated. We
      conclude that Tmp21-II cDNA derives from a neutral pseudogene, which originates
      from a duplication event of the human Tmp21-isoform-I.
FAU - Horer, J
AU  - Horer J
AD  - Institute of Physiology II, University of the Saarland, Saar, Germany.
FAU - Blum, R
AU  - Blum R
FAU - Feick, P
AU  - Feick P
FAU - Nastainczyk, W
AU  - Nastainczyk W
FAU - Schulz, I
AU  - Schulz I
LA  - eng
SI  - GENBANK/AJ004912
SI  - GENBANK/AJ004913
SI  - GENBANK/AJ004914
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Seq
JT  - DNA sequence : the journal of DNA sequencing and mapping
JID - 9107800
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (TMED10 protein, human)
RN  - 0 (TMED10P1 protein, human)
RN  - 0 (Tmed10 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cricetinae
MH  - DNA, Complementary
MH  - Humans
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Nucleocytoplasmic Transport Proteins
MH  - Protein Isoforms/genetics
MH  - Rats
EDAT- 1999/06/22 10:00
MHDA- 2000/08/12 11:00
CRDT- 1999/06/22 10:00
PHST- 1999/06/22 10:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/06/22 10:00 [entrez]
AID - I307J981055 [pii]
AID - 10.3109/10425179909008429 [doi]
PST - ppublish
SO  - DNA Seq. 1999;10(2):121-6. doi: 10.3109/10425179909008429.