PMID- 10375619
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20191008
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 233
IP  - 1-2
DP  - 1999 Jun 11
TI  - Characterization of the components of the putative mammalian sister chromatid
      cohesion complex.
PG  - 39-47
AB  - Establishing and maintaining proper sister chromatid cohesion throughout the cell
      cycle are essential for maintaining genome integrity. To understand how sister
      chromatid cohesion occurs in mammals, we have cloned and characterized mouse
      orthologs of proteins known to be involved in sister chromatid cohesion in other 
      organisms. The cDNAs for the mouse orthologs of SMC1S.c. and SMC3S.c. , mSMCB and
      mSMCD respectively, were cloned, and the corresponding transcripts and proteins
      were characterized. mSMCB and mSMCD are transcribed at similar levels in adult
      mouse tissues except in testis, which has an excess of mSMCD transcripts. The
      mSMCB and mSMCD proteins, as well as the PW29 protein, a mouse homolog of
      Mcd1pS.c./Rad21S.p., form a complex similar to cohesin in X. laevis. mSMCB, mSMCD
      and PW29 protein levels show no significant cell-cycle dependence. The bulk of
      the mSMCB, mSMCD and PW29 proteins undergo redistribution from the chromosome
      vicinity to the cytoplasm during prometaphase and back to the chromatin in
      telophase. This pattern of intracellular localization suggests a complex role for
      this group of SMC proteins in chromosome dynamics. The PW29 protein and PCNA,
      which have both been implicated in sister chromatid cohesion, do not colocalize, 
      indicating that these proteins may not function in the same cohesion pathway.
      Overexpression of a PW29-GFP fusion protein in mouse fibroblasts leads to
      inhibition of proliferation, implicating this protein and its complex with SMC
      proteins in the control of mitotic cycle progression.
FAU - Darwiche, N
AU  - Darwiche N
AD  - Unit of Chromosome Structure and Function, NIH, NICHD, Laboratory of Molecular
      Embryology, 18T Library Drive, room 106, Bethesda, MD 20892-5430, USA.
FAU - Freeman, L A
AU  - Freeman LA
FAU - Strunnikov, A
AU  - Strunnikov A
LA  - eng
SI  - GENBANK/AF047600
SI  - GENBANK/AF047601
GR  - Z01 HD001903-11/Intramural NIH HHS/United States
GR  - Z99 AI999999/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Cspg6 protein, mouse)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (structural maintenance of chromosome protein 1)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle
MH  - Cell Cycle Proteins/*genetics
MH  - *Chondroitin Sulfate Proteoglycans
MH  - Chromatids/*genetics
MH  - Chromosomal Proteins, Non-Histone/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Fungal Proteins/*genetics
MH  - Mice
MH  - Mitosis
MH  - Molecular Sequence Data
PMC - PMC2670183
MID - NIHMS101852
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0378111999001602 [pii]
AID - 10.1016/s0378-1119(99)00160-2 [doi]
PST - ppublish
SO  - Gene. 1999 Jun 11;233(1-2):39-47. doi: 10.1016/s0378-1119(99)00160-2.