PMID- 10375507
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20071114
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 126
IP  - 14
DP  - 1999 Jun
TI  - UNC-84 localizes to the nuclear envelope and is required for nuclear migration
      and anchoring during C. elegans development.
PG  - 3171-81
AB  - Nuclear migrations are essential for metazoan development. Two nuclear migrations
      that occur during C. elegans development require the function of the unc-84 gene.
      unc-84 mutants are also defective in the anchoring of nuclei within the
      hypodermal syncytium and in the migrations of the two distal tip cells of the
      gonad. Complementation analyses of 17 unc-84 alleles defined two genetically
      separable functions. Both functions are required for nuclear and distal tip cell 
      migrations, but only one is required for nuclear anchorage. The DNA lesions
      associated with these 17 mutations indicate that the two genetically defined
      functions correspond to two distinct regions of the UNC-84 protein. The UNC-84
      protein has a predicted transmembrane domain and a C-terminal region with
      similarity to the S. pombe spindle pole body protein Sad1 and to two predicted
      mammalian proteins. Analysis of a green fluorescent protein reporter indicated
      that UNC-84 is widely expressed and localized to the nuclear envelope. We propose
      that UNC-84 functions to facilitate a nuclear-centrosomal interaction required
      for nuclear migration and anchorage.
FAU - Malone, C J
AU  - Malone CJ
AD  - Howard Hughes Medical Institute, Department of Molecular, Cellular and
      Developmental Biology, University of Colorado at Boulder, Boulder CO 80309-0347, 
      USA.
FAU - Fixsen, W D
AU  - Fixsen WD
FAU - Horvitz, H R
AU  - Horvitz HR
FAU - Han, M
AU  - Han M
LA  - eng
SI  - GENBANK/AF200706
SI  - GENBANK/AF200707
SI  - GENBANK/AF202723
SI  - GENBANK/AF202724
GR  - GM24663/GM/NIGMS NIH HHS/United States
GR  - GM47869/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Unc-84 protein, C elegans)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - Caenorhabditis elegans/genetics/*growth & development
MH  - *Caenorhabditis elegans Proteins
MH  - Cell Nucleus/genetics/*metabolism
MH  - Conserved Sequence
MH  - Genetic Complementation Test
MH  - Helminth Proteins/*genetics/*metabolism
MH  - Larva/cytology/genetics/growth & development
MH  - Membrane Glycoproteins/*genetics/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Envelope/genetics/metabolism
MH  - Nuclear Proteins/*genetics/metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
PST - ppublish
SO  - Development. 1999 Jun;126(14):3171-81.