PMID- 10375506
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190514
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 126
IP  - 14
DP  - 1999 Jun
TI  - XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development.
PG  - 3159-70
AB  - XTcf-3 is an HMG box transcription factor that mediates Xenopus dorsal-ventral
      axis formation. As a Wnt pathway effector, XTcf-3 interacts with beta-catenin and
      activates the expression of the dorsal organizing gene siamois, while in the
      absence of beta-catenin, XTcf-3 functions as a transcriptional repressor. We show
      that XTcf-3 contains amino- and carboxy-terminal repressor domains and have
      identified a Xenopus member of the C-terminal Binding Protein family of
      transcriptional co-repressors (XCtBP) as the C-terminal co-repressor. We show
      that two XCtBP binding sites near the XTcf-3 carboxy-terminus are required for
      the interaction of XTcf-3 and XCtBP and for the transcriptional repression
      mediated by the XTcf-3 carboxy-terminal domain. By fusing the GAL4 activation
      domain to XCtBP we have generated an antimorphic protein, XCtBP/G4A, that
      activates siamois transcription through an interaction with endogenous XTcf-3.
      Ectopic expression of XCtBP/G4A demonstrates that XCtBP functions in the
      regulation of head and notochord development. Our data support a role for XCtBP
      as a co-repressor throughout Xenopus development and indicate that XCtBP/G4A will
      be a useful tool in determining how XCtBP functions in various developmental
      processes.
FAU - Brannon, M
AU  - Brannon M
AD  - Department of Biochemistry, Howard Hughes Medical Institute, Center for
      Developmental Biology, University of Washington School of Medicine, Seattle,
      Washington 98195-7350, USA. kimelman@u.washington.edu.
FAU - Brown, J D
AU  - Brown JD
FAU - Bates, R
AU  - Bates R
FAU - Kimelman, D
AU  - Kimelman D
FAU - Moon, R T
AU  - Moon RT
LA  - eng
SI  - GENBANK/AF152006
GR  - 2T32HD07183-16/HD/NICHD NIH HHS/United States
GR  - 5T32HD07183-17/HD/NICHD NIH HHS/United States
GR  - HD27262/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Carrier Proteins)
RN  - 0 (HMGB Proteins)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Lef1 protein, Xenopus)
RN  - 0 (Lymphoid Enhancer-Binding Factor 1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SIA1 protein, Xenopus)
RN  - 0 (TCF Transcription Factors)
RN  - 0 (Tcf3 protein, Xenopus)
RN  - 0 (Thyroid Hormones)
RN  - 0 (Transcription Factor 3)
RN  - 0 (Transcription Factor 7-Like 1 Protein)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
RN  - 0 (thyroid hormone-binding proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Conserved Sequence
MH  - Embryo, Nonmammalian
MH  - *Gene Expression Regulation, Developmental
MH  - *HMGB Proteins
MH  - High Mobility Group Proteins/genetics/metabolism
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Hybrid Cells
MH  - Lymphoid Enhancer-Binding Factor 1
MH  - Membrane Proteins/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/genetics/metabolism
MH  - Repressor Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - TCF Transcription Factors
MH  - *Thyroid Hormones
MH  - Transcription Factor 3
MH  - Transcription Factor 7-Like 1 Protein
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Xenopus/*embryology/genetics
MH  - *Xenopus Proteins
MH  - Yeasts/genetics
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
PST - ppublish
SO  - Development. 1999 Jun;126(14):3159-70.