PMID- 10375449
OWN - NLM
STAT- MEDLINE
DCOM- 19990730
LR  - 20161124
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 69
IP  - 1
DP  - 1999 Jul
TI  - Characterization of a novel C-kinesin (KIFC3) abundantly expressed in vertebrate 
      retina and RPE.
PG  - 57-68
AB  - Many forms of intracellular transport are mediated by microtubule-dependent
      motors of the kinesin superfamily (KIFs). To identify kinesins expressed in human
      retina and RPE, we used degenerate primer RT-PCR to amplify a approximately 440
      bp kinesin motor domain fragment from human retinal and RPE messenger RNAs. Four 
      distinct kinesins were detected: one C-kinesin (HsKIFC3); one kinesin from the
      unc104/KIF1 family [HsKIF1A]; and the ubiquitous and neuronal forms of
      conventional kinesin heavy chain [HsuKHC and HsnKHC]. The C-kinesin HsKIFC3
      comprised 33.3% of the retinal clones and was 60% identical to FKIF2, the most
      abundant kinesin detected in a previous screen of fish retina and 95% identical
      to a fragment of MmKifC3 recently amplified from mouse brain. Elsewhere we have
      reported the sequence of HsKIFC3 and shown that it maps to the same locus on
      chromosome 16q13-q21 as Bardet-Biedl syndrome Type II, a hereditary retinal
      degeneration. We describe here the kinesin PCR screen of human retina and RPE and
      examine the tissue and subcellular distribution of KIFC3 in both fish and human
      retina using an antibody raised against a peptide conserved between FKIF2 and
      HsKIFC3. This peptide antibody identified a single approximately 80 kDa band in
      Western blots of fish and human retina and RPE. In both fish and human retina
      this antibody strongly labeled photoreceptor terminals in the outer plexiform
      layer, suggesting that FKIF2/KIFC3 may play some role in the photoreceptor
      synapse.
CI  - Copyright 1999 Academic Press.
FAU - Hoang, E
AU  - Hoang E
AD  - Department of Molecular and Cell Biology, University of California, 335 Life
      Sciences Addition, Berkeley, CA, 94720-3200, USA.
FAU - Bost-Usinger, L
AU  - Bost-Usinger L
FAU - Burnside, B
AU  - Burnside B
LA  - eng
GR  - EY03575/EY/NEI NIH HHS/United States
GR  - EY6534/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (KIF3C protein, human)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bass/*metabolism
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Cytoskeleton/metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Kinesin/chemistry/immunology/*metabolism
MH  - Molecular Sequence Data
MH  - Photoreceptor Cells, Vertebrate/metabolism
MH  - Pigment Epithelium of Eye/*metabolism
MH  - Retina/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1006/exer.1999.0671 [doi]
AID - S0014-4835(99)90671-1 [pii]
PST - ppublish
SO  - Exp Eye Res. 1999 Jul;69(1):57-68. doi: 10.1006/exer.1999.0671.