PMID- 10375378
OWN - NLM
STAT- MEDLINE
DCOM- 19990726
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 47
IP  - 7
DP  - 1999 Jul
TI  - Differential expression of the cyclic GMP-stimulated phosphodiesterase PDE2A in
      human venous and capillary endothelial cells.
PG  - 895-906
AB  - We developed selective monoclonal antibodies and used them for Western and
      immunocytochemical analyses to determine the tissue and cellular distribution of 
      the human cyclic GMP-stimulated phosphodiesterase (PDE2). Western analysis
      revealed PDE2A expression in a variety of tissue types, including cerebellum,
      neocortex, heart, kidney, lung, pulmonary artery, and skeletal muscle.
      Immunocytochemical analysis revealed PDE2A expression in a subset of tissue
      endothelial cells. PDE2A immunostaining was detected in venous and capillary
      endothelial cells in cardiac and renal tissue but not in arterial endothelial
      cells. These results were confirmed by in situ hybridization. PDE2A
      immunostaining was also absent from luminal endothelial cells of large vessels,
      such as aorta, pulmonary, and renal arteries, but was present in the endothelial 
      cells of the vasa vasorum. PDE2A immunostaining was detected in the endothelial
      cells of a variety of microvessels, including those in renal and cardiac
      interstitial spaces, renal glomerulus, skin, brain, and liver. Although PDE2A was
      not readily detected in arterial endothelial cells by immunocytochemistry of
      intact tissue, it was detected at low levels in cultured arterial endothelial
      cells. These results suggest a possible role for PDE2A in modulating the effects 
      of cyclic nucleotides on fluid and inflammatory cell transit through the
      endothelial cell barrier.
FAU - Sadhu, K
AU  - Sadhu K
AD  - ICOS Corporation, Bothell, Washington, 98021, USA.
FAU - Hensley, K
AU  - Hensley K
FAU - Florio, V A
AU  - Florio VA
FAU - Wolda, S L
AU  - Wolda SL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the
      Histochemistry Society
JID - 9815334
RN  - 0 (von Willebrand Factor)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
SB  - IM
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/*biosynthesis
MH  - Blotting, Western
MH  - Capillaries/metabolism
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Tissue Distribution
MH  - Veins/metabolism
MH  - von Willebrand Factor/metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1177/002215549904700707 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1999 Jul;47(7):895-906. doi: 10.1177/002215549904700707.