PMID- 10373557
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 7
DP  - 1999 Jul
TI  - A novel growth- and cell cycle-regulated protein, ASK, activates human
      Cdc7-related kinase and is essential for G1/S transition in mammalian cells.
PG  - 5083-95
AB  - A novel human protein, ASK (activator of S phase kinase), was identified on the
      basis of its ability to bind to human Cdc7-related kinase (huCdc7). ASK forms an 
      active kinase complex with huCdc7 that is capable of phosphorylating MCM2
      protein. ASK appears to be the major activator of huCdc7, since immunodepletion
      of ASK protein from the extract is accompanied by the loss of huCdc7-dependent
      kinase activity. Expression of ASK is regulated by growth factor stimulation, and
      levels oscillate through the cell cycle, reaching a peak during S phase.
      Concomitantly, the huCdc7-dependent kinase activity significantly increases when 
      cells are in S phase. Furthermore, we have demonstrated that ASK serves an
      essential function for entry into S phase by showing that microinjection of
      ASK-specific antibodies into mammalian cells inhibited DNA replication. Our data 
      show that ASK is a novel cyclin-like regulatory subunit of the huCdc7 kinase
      complex and that it plays a pivotal role in G1/S transition in mammalian cells.
FAU - Kumagai, H
AU  - Kumagai H
AD  - Department of Molecular and Developmental Biology, Institute of Medical Science, 
      University of Tokyo, Japan.
FAU - Sato, N
AU  - Sato N
FAU - Yamada, M
AU  - Yamada M
FAU - Mahony, D
AU  - Mahony D
FAU - Seghezzi, W
AU  - Seghezzi W
FAU - Lees, E
AU  - Lees E
FAU - Arai, K
AU  - Arai K
FAU - Masai, H
AU  - Masai H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (CDC7 protein, human)
RN  - EC 2.7.1.- (Cdc7 protein, mouse)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle
MH  - Cell Cycle Proteins/genetics/*metabolism/*physiology
MH  - Cell Division
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Enzyme Activation
MH  - G1 Phase/*physiology
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Kinases/*metabolism
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - RNA, Messenger
MH  - S Phase/*physiology
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
PMC - PMC84351
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1128/mcb.19.7.5083 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jul;19(7):5083-95. doi: 10.1128/mcb.19.7.5083.