PMID- 10373521
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 7
DP  - 1999 Jul
TI  - Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a
      TATA-box-dependent manner.
PG  - 4719-28
AB  - Tat protein strongly activates transcription from the human immunodeficiency
      virus type 1 (HIV-1) long terminal repeat (LTR) by enhancing the elongation
      efficiency of RNA polymerase II complexes. Tat-mediated transcriptional
      activation requires cellular cofactors and specific cis-acting elements within
      the HIV-1 promoter, among them a functional TATA box. Here, we have investigated 
      the mechanism by which one of these cofactors, termed CA150, regulates HIV-1
      transcription in vivo. We present a series of functional assays that demonstrate 
      that the regulation of the HIV-1 LTR by CA150 has the same functional
      requirements as the activation by Tat. We found that CA150 affects elongation of 
      transcription complexes assembled on the HIV-1 promoter in a TATA-box-dependent
      manner. We discuss the data in terms of the involvement of CA150 in the
      regulation of Tat-activated HIV-1 gene expression. In addition, we also provide
      evidence suggesting a role for CA150 in the regulation of cellular
      transcriptional processes.
FAU - Sune, C
AU  - Sune C
AD  - Departments of Pharmacology and Cancer Biology, Levine Science Research Center,
      Duke University Medical Center, Durham, North Carolina 27710, USA.
FAU - Garcia-Blanco, M A
AU  - Garcia-Blanco MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gene Products, tat)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (TCERG1 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
SB  - X
MH  - Cell Line, Transformed
MH  - DNA-Binding Proteins/genetics
MH  - *Gene Expression Regulation, Viral
MH  - Gene Products, tat/metabolism
MH  - *HIV Long Terminal Repeat
MH  - HIV-1/*genetics
MH  - Humans
MH  - Promoter Regions, Genetic
MH  - RNA Polymerase II/*metabolism
MH  - *TATA Box
MH  - TATA-Box Binding Protein
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors/genetics
MH  - Transcription, Genetic
MH  - Transcriptional Elongation Factors
MH  - tat Gene Products, Human Immunodeficiency Virus
PMC - PMC84270
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1128/mcb.19.7.4719 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jul;19(7):4719-28. doi: 10.1128/mcb.19.7.4719.