PMID- 10373512
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 7
DP  - 1999 Jul
TI  - Interaction between the product of the breast cancer susceptibility gene BRCA2
      and DSS1, a protein functionally conserved from yeast to mammals.
PG  - 4633-42
AB  - Germ line mutations in the breast cancer susceptibility gene BRCA2 predispose to 
      early-onset breast cancer, but the function of the nuclear protein encoded by the
      gene is ill defined. Using the yeast two-hybrid system with fragments of human
      BRCA2, we identified an interaction with the human DSS1 (deleted in split
      hand/split foot) gene. Yeast and mammalian two-hybrid assays showed that DSS1 can
      associate with BRCA2 in the region of amino acids 2472 to 2957 in the C terminus 
      of the protein. Using coimmunoprecipitation of epitope-tagged BRCA2 and DSS1 cDNA
      constructs transiently expressed in COS cells, we were able to demonstrate an
      association. Furthermore, endogenous BRCA2 could be coimmunoprecipitated with
      endogenous DSS1 in MCF7 cells, demonstrating an in vivo association. Apparent
      orthologues of the mammalian DSS1 gene were identified in the genome of the
      yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. Yeast strains in
      which these DSS1-like genes were deleted showed a temperature-sensitive growth
      phenotype, which was analyzed by flow cytometry. This provides evidence for a
      link between the BRCA2 tumor suppressor gene and a gene required for completion
      of the cell cycle.
FAU - Marston, N J
AU  - Marston NJ
AD  - Section of Gene Function and Regulation, Institute of Cancer Research, Chester
      Beatty Laboratories, London, United Kingdom SW3 6JB.
FAU - Richards, W J
AU  - Richards WJ
FAU - Hughes, D
AU  - Hughes D
FAU - Bertwistle, D
AU  - Bertwistle D
FAU - Marshall, C J
AU  - Marshall CJ
FAU - Ashworth, A
AU  - Ashworth A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (BRCA2 Protein)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SEM1 protein, human)
RN  - 0 (Shfdg1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - BRCA2 Protein
MH  - Breast Neoplasms/genetics/*metabolism
MH  - COS Cells
MH  - Cell Cycle Proteins
MH  - Cloning, Molecular
MH  - *Conserved Sequence
MH  - Female
MH  - Genes, Fungal
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Precipitin Tests
MH  - Proteasome Endopeptidase Complex
MH  - Proteins/genetics/*metabolism
MH  - Rabbits
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae/*genetics
MH  - Schizosaccharomyces/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Temperature
MH  - Transcription Factors/genetics/*metabolism
MH  - Tumor Cells, Cultured
PMC - PMC84261
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1128/MCB.19.7.4633 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Jul;19(7):4633-42. doi: 10.1128/MCB.19.7.4633.