PMID- 10373493
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - Analysis of tyrosine phosphorylation-dependent interactions between stimulatory
      effector proteins and the B cell co-receptor CD22.
PG  - 18769-76
AB  - The B cell-restricted transmembrane glycoprotein CD22 is rapidly phosphorylated
      on tyrosine in response to cross-linking of the B cell antigen receptor, thereby 
      generating phosphotyrosine motifs in the cytoplasmic domain which recruit
      intracellular effector proteins that contain Src homology 2 domains. By virtue of
      its interaction with these effector proteins CD22 modulates signal transduction
      through the B cell antigen receptor. To define further the molecular mechanism by
      which CD22 mediates its co-receptor function, phosphopeptide mapping experiments 
      were conducted to determine which of the six tyrosine residues in the cytoplasmic
      domain are involved in recruitment of the stimulatory effector proteins
      phospholipase Cgamma (PLCgamma), phosphoinositide 3-kinase (PI3K), Grb2, and Syk.
      The results obtained indicate that the protein tyrosine kinase Syk interacts with
      multiple CD22-derived phosphopeptides in both immunoprecipitation and reverse Far
      Western assays. In contrast, the Grb2.Sos complex was observed to bind
      exclusively to the fourth phosphotyrosine motif (Y828ENV) from CD22 and does so
      via a direct interaction based on Far Western and reverse Far Western blotting.
      Although both PLCgamma and PI3K were observed to bind to multiple phosphopeptides
      in precipitation experiments, subsequent studies using reverse Far Western blot
      analysis demonstrated that only the carboxyl-terminal phosphopeptide of CD22
      (Y863VTL) binds directly to either one. This finding suggests that PLCgamma and
      PI3K may be recruited to CD22 either through a direct interaction with Tyr863 or 
      indirectly through an association with one or more intermediate proteins.
FAU - Yohannan, J
AU  - Yohannan J
AD  - Department of Microbiology and Immunology, University of Texas Medical Branch,
      Galveston, Texas 77555, USA.
FAU - Wienands, J
AU  - Wienands J
FAU - Coggeshall, K M
AU  - Coggeshall KM
FAU - Justement, L B
AU  - Justement LB
LA  - eng
GR  - AI36401/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD22 protein, human)
RN  - 0 (Cd22 protein, mouse)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Enzyme Precursors)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (GRB2 protein, human)
RN  - 0 (Grb2 protein, mouse)
RN  - 0 (Grb2 protein, rat)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Lectins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 2)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
RN  - EC 2.7.10.2 (Syk protein, rat)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Antigens, Differentiation, B-Lymphocyte/*metabolism
MH  - B-Lymphocytes/*metabolism
MH  - Binding Sites
MH  - Cattle
MH  - *Cell Adhesion Molecules
MH  - Cytoplasm/metabolism
MH  - Enzyme Precursors/*metabolism
MH  - GRB2 Adaptor Protein
MH  - Guanine Nucleotide Exchange Factors
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Isoenzymes/*metabolism
MH  - *Lectins
MH  - Mice
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Proteins/metabolism
MH  - Rabbits
MH  - Rats
MH  - Receptors, Antigen, B-Cell/metabolism
MH  - Sialic Acid Binding Ig-like Lectin 2
MH  - Signal Transduction
MH  - Syk Kinase
MH  - Type C Phospholipases/*metabolism
MH  - Tyrosine/*metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18769 [doi]
AID - S0021-9258(19)87219-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18769-76. doi: 10.1074/jbc.274.26.18769.