PMID- 10373470
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - The cloning and analysis of LEK1 identifies variations in the LEK/centromere
      protein F/mitosin gene family.
PG  - 18597-604
AB  - We report the cloning of a novel murine cDNA, LEK1, that is related to human
      CENP-F and mitosin and more distantly to chicken CMF1. The proteins from these
      three organisms have significant homology, yet differ in their temporal, spatial,
      and subcellular localizations. The human proteins bind the kinetochore in mitotic
      cells, whereas the chicken protein is found only in skeletal and cardiac muscle
      and is developmentally regulated. Mouse LEK1 is a single copy gene that codes for
      two developmentally regulated transcripts. The LEK1 protein is expressed early
      and ubiquitously in mouse development and is generally down-regulated as
      development proceeds in a manner that correlates to a cessation of mitosis. In
      adult tissues, the LEK1 protein is detected exclusively in the pronucleus of the 
      oocyte and was not observed in other actively dividing tissues. Subcellular
      localization revealed that the LEK1 protein in mitotic cells does not bind the
      kinetochore. From these data, we hypothesize that chicken CMF1, human CENP-F,
      mitosin, and mouse LEK1 are members of an emerging family of genes that have
      important and functionally distinct roles in development and cell division.
FAU - Goodwin, R L
AU  - Goodwin RL
AD  - Gladys P. Stahlman Cardiovascular Research Laboratory, Vanderbilt University
      Medical Center, Nashville, Tennessee 37212-6300, USA.
FAU - Pabon-Pena, L M
AU  - Pabon-Pena LM
FAU - Foster, G C
AU  - Foster GC
FAU - Bader, D
AU  - Bader D
LA  - eng
SI  - GENBANK/AF194970
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - HL09916/HL/NHLBI NIH HHS/United States
GR  - HL37675/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Autoantigens)
RN  - 0 (Avian Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Complementary)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (cardiac muscle factor 1 protein, chicken)
RN  - 0 (centromere protein F)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantigens/*genetics
MH  - *Avian Proteins
MH  - Cell Differentiation
MH  - Centromere/*genetics
MH  - Chickens
MH  - Chromosomal Proteins, Non-Histone/*chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Humans
MH  - Mice
MH  - Microfilament Proteins
MH  - Mitosis/genetics
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Muscle Proteins/*chemistry
MH  - Muscle, Skeletal/embryology
MH  - Nuclear Proteins/*chemistry
MH  - Phosphoproteins/*chemistry
MH  - RNA, Messenger/metabolism
MH  - Sequence Analysis, DNA
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18597 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18597-604. doi: 10.1074/jbc.274.26.18597.