PMID- 10373468
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - GPI1 stabilizes an enzyme essential in the first step of
      glycosylphosphatidylinositol biosynthesis.
PG  - 18582-8
AB  - Attachment of glycosylphosphatidylinositol (GPI) is essential for the surface
      expression of many proteins. Biosynthesis of glycosylphosphatidylinositol is
      initiated by the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to 
      phosphatidylinositol. In mammalian cells, this reaction is mediated by a complex 
      of PIG-A, PIG-H, PIG-C, and GPI1. This complexity may be relevant for regulation 
      and for usage of a particular phosphatidylinositol. However, the functions of the
      respective components have been unclear. Here we cloned the mouse GPI1 gene and
      disrupted it in F9 embryonal carcinoma cells. Disruption of the GPI1 gene caused 
      a severe but not complete defect in the generation of
      glycosylphosphatidylinositol-anchored proteins, indicating some residual
      biosynthetic activity. A complex of PIG-A, PIG-H, and PIG-C decreased to a nearly
      undetectable level, whereas a complex of PIG-A and PIG-H was easily detected. A
      lack of GPI1 also caused partial decreases of PIG-C and PIG-H. Therefore, GPI1
      stabilizes the enzyme by tying up PIG-C with a complex of PIG-A and PIG-H.
FAU - Hong, Y
AU  - Hong Y
AD  - Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka 
      University, Osaka 565-0871, Japan.
FAU - Ohishi, K
AU  - Ohishi K
FAU - Watanabe, R
AU  - Watanabe R
FAU - Endo, Y
AU  - Endo Y
FAU - Maeda, Y
AU  - Maeda Y
FAU - Kinoshita, T
AU  - Kinoshita T
LA  - eng
SI  - GENBANK/AB008915
SI  - GENBANK/AB008916
SI  - GENBANK/AB008917
SI  - GENBANK/AB008918
SI  - GENBANK/AB008919
SI  - GENBANK/AB008920
SI  - GENBANK/AB008921
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Membrane Proteins)
RN  - 0 (PIGQ protein, human)
RN  - 0 (Pigq protein, mouse)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Enzyme Stability
MH  - Glycosylphosphatidylinositols/*biosynthesis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - N-Acetylglucosaminyltransferases/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18582 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18582-8. doi: 10.1074/jbc.274.26.18582.