PMID- 10373455
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - Specific expression of activation-induced cytidine deaminase (AID), a novel
      member of the RNA-editing deaminase family in germinal center B cells.
PG  - 18470-6
AB  - We have identified a novel gene referred to as activation-induced deaminase (AID)
      by subtraction of cDNAs derived from switch-induced and uninduced murine B
      lymphoma CH12F3-2 cells, more than 80% of which switch exclusively to IgA upon
      stimulation. The amino acid sequence encoded by AID cDNA is homologous to that of
      apolipoprotein B (apoB) mRNA-editing enzyme, catalytic polypeptide 1 (APOBEC-1), 
      a type of cytidine deaminase that constitutes a catalytic subunit for the apoB
      mRNA-editing complex. In vitro experiments using a glutathione S-transferase AID 
      fusion protein revealed significant cytidine deaminase activity that is blocked
      by tetrahydrouridine and by zinc chelation. However, AID alone did neither
      demonstrate activity in C to U editing of apoB mRNA nor bind to AU-rich RNA
      targets. AID mRNA expression is induced in splenic B cells that were activated in
      vitro or by immunizations with sheep red blood cells. In situ hybridization of
      immunized spleen sections revealed the restricted expression of AID mRNA in
      developing germinal centers in which modulation of immunoglobulin gene
      information through somatic hypermutation and class switch recombination takes
      place. Taken together, these findings suggest that AID is a new member of the
      RNA-editing deaminase family and may play a role in genetic events in the
      germinal center B cell.
FAU - Muramatsu, M
AU  - Muramatsu M
AD  - Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Yoshida, 
      Sakyo-ku, Kyoto 606-8501, Japan.
FAU - Sankaranand, V S
AU  - Sankaranand VS
FAU - Anant, S
AU  - Anant S
FAU - Sugai, M
AU  - Sugai M
FAU - Kinoshita, K
AU  - Kinoshita K
FAU - Davidson, N O
AU  - Davidson NO
FAU - Honjo, T
AU  - Honjo T
LA  - eng
SI  - GENBANK/AF132979
GR  - DK 42086/DK/NIDDK NIH HHS/United States
GR  - HL 38180/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Apolipoproteins B)
RN  - 0 (DNA, Complementary)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.5.4.- (AICDA (activation-induced cytidine deaminase))
RN  - EC 3.5.4.36 (APOBEC-1 Deaminase)
RN  - EC 3.5.4.36 (Apobec1 protein, mouse)
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
MH  - APOBEC-1 Deaminase
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apolipoproteins B
MH  - B-Lymphocytes/*enzymology
MH  - Cycloheximide/pharmacology
MH  - Cytidine Deaminase/*biosynthesis/chemistry/genetics
MH  - DNA, Complementary/isolation & purification
MH  - Enzyme Induction/drug effects
MH  - Gene Library
MH  - Germinal Center/*cytology/enzymology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - *RNA Editing
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
MH  - Tumor Cells, Cultured
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18470 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18470-6. doi: 10.1074/jbc.274.26.18470.