PMID- 10373451
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - Cloning and characterization of a novel human phosphodiesterase that hydrolyzes
      both cAMP and cGMP (PDE10A).
PG  - 18438-45
AB  - cDNA encoding a novel phosphodiesterase (PDE) was isolated from a human fetal
      lung cDNA library and designated PDE10A. The deduced amino acid sequence contains
      779 amino acids, including a putative cGMP binding sequence in the amino-terminal
      portion of the molecule and a catalytic domain that is 16-47% identical in amino 
      acid sequence to those of other PDE families. Recombinant PDE10A transfected and 
      expressed in COS-7 cells hydrolyzed cAMP and cGMP with Km values of 0.26 and 7.2 
      microM, respectively, and Vmax with cGMP was almost twice that with cAMP. Of the 
      PDE inhibitors tested, dipyridamole was most effective, with IC50 values of 1.2
      and 0.45 microM for inhibition of cAMP and cGMP hydrolysis, respectively. cGMP
      inhibited hydrolysis of cAMP, and cAMP inhibited cGMP hydrolysis with IC50 values
      of 14 and 0.39 microM, respectively. Thus, PDE10A exhibited properties of a cAMP 
      PDE and a cAMP-inhibited cGMP PDE. PDE10A transcripts were particularly abundant 
      in the putamen and caudate nucleus regions of brain and in thyroid and testis,
      and in much lower amounts in other tissues. The PDE10A gene was located on
      chromosome 6q26 by fluorescent in situ hybridization analysis. PDE10A represents 
      a new member of the PDE superfamily, exhibiting unique kinetic properties and
      inhibitor sensitivity.
FAU - Fujishige, K
AU  - Fujishige K
AD  - Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd., 2-50, Kawagishi-2-chome, 
      Toda, Saitama 335-8505, Japan.
FAU - Kotera, J
AU  - Kotera J
FAU - Michibata, H
AU  - Michibata H
FAU - Yuasa, K
AU  - Yuasa K
FAU - Takebayashi, S
AU  - Takebayashi S
FAU - Okumura, K
AU  - Okumura K
FAU - Omori, K
AU  - Omori K
LA  - eng
SI  - GENBANK/AB020593
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.1.4.- (PDE10A protein, human)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - Catalytic Domain
MH  - *Chromosomes, Human, Pair 6
MH  - Cloning, Molecular
MH  - Cyclic AMP/*metabolism
MH  - Cyclic GMP/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Phosphoric Diester Hydrolases/*genetics/isolation & purification/metabolism
MH  - Transfection
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18438 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18438-45. doi: 10.1074/jbc.274.26.18438.