PMID- 10373431
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - Identification of TATA-binding protein-free TAFII-containing complex subunits
      suggests a role in nucleosome acetylation and signal transduction.
PG  - 18285-9
AB  - Recently we identified a novel human (h) multiprotein complex, called
      TATA-binding protein (TBP)-free TAFII-containing complex (TFTC), which is able to
      nucleate RNA polymerase II transcription and can mediate transcriptional
      activation. Here we demonstrate that TFTC, similar to other TBP-free TAFII
      complexes (yeast SAGA, hSTAGA, and hPCAF) contains the acetyltransferase hGCN5
      and is able to acetylate histones in both a free and a nucleosomal context. The
      recently described TRRAP cofactor for oncogenic transcription factor pathways was
      also characterized as a TFTC subunit. Furthermore, we identified four other
      previously uncharacterized subunits of TFTC: hADA3, hTAFII150, hSPT3, and
      hPAF65beta. Thus, the polypeptide composition of TFTC suggests that TFTC is
      recruited to chromatin templates by activators to acetylate histones and thus may
      potentiate initiation and activation of transcription.
FAU - Brand, M
AU  - Brand M
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire,
      CNRS/INSERM/Universite Louis Pasteur BP 163-67404 Illkirch Cedex, CU de
      Strasbourg, France.
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Staub, A
AU  - Staub A
FAU - Tora, L
AU  - Tora L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (NGG1 protein, S cerevisiae)
RN  - 0 (Nucleosomes)
RN  - 0 (SUPT3H protein, human)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (TAF2 protein, human)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
RN  - EC 2.3.1.48 (p300-CBP-associated factor)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Acetylation
MH  - Acetyltransferases/metabolism
MH  - Carrier Proteins/metabolism
MH  - Cell Cycle Proteins
MH  - DNA-Binding Proteins/metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Macromolecular Substances
MH  - Multiprotein Complexes
MH  - Nucleosomes/*metabolism
MH  - RNA Polymerase II/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - *Signal Transduction
MH  - *TATA-Binding Protein Associated Factors
MH  - TATA-Box Binding Protein
MH  - Trans-Activators/metabolism
MH  - *Transcription Factor TFIID
MH  - Transcription Factors/metabolism/*physiology
MH  - Transcription Factors, TFII/*physiology
MH  - Transcriptional Activation
MH  - p300-CBP Transcription Factors
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18285 [doi]
AID - S0021-9258(19)87157-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18285-9. doi: 10.1074/jbc.274.26.18285.