PMID- 10373416
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - CAST, a novel CD3epsilon-binding protein transducing activation signal for
      interleukin-2 production in T cells.
PG  - 18173-80
AB  - Antigen recognition through T cell receptor (TCR)-CD3 complex transduces signals 
      into T cells, which regulate activation, function, and differentiation of T
      cells. The TCR-CD3 complex is composed of two signaling modules represented by
      CD3zeta and CD3epsilon. Signaling through CD3zeta has been extensively analyzed, 
      but that via CD3epsilon, which is also crucial in immature thymocyte development,
      is still not clearly understood. We isolated cDNA encoding a novel
      CD3epsilon-binding protein CAST. CAST specifically interacts in vivo and in vitro
      with CD3epsilon but not with CD3zeta or FcRgamma via a unique membrane-proximal
      region of CD3epsilon. CAST is composed of 512 amino acids including a single
      tyrosine and undergoes tyrosine phosphorylation upon TCR stimulation.
      Overexpression of two dominant-negative types of CAST, a minimum
      CD3epsilon-binding domain and a tyrosine-mutant, strongly suppressed NFAT
      activation and interleukin-2 production. These results demonstrate that CAST
      serves as a component of preformed TCR complex and transduces activation signals 
      upon TCR stimulation and represents a new signaling pathway via the
      CD3epsilon-containing TCR signaling module.
FAU - Yamazaki, T
AU  - Yamazaki T
AD  - Department of Molecular Genetics, Chiba University Graduate School of Medicine,
      1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
FAU - Hamano, Y
AU  - Hamano Y
FAU - Tashiro, H
AU  - Tashiro H
FAU - Itoh, K
AU  - Itoh K
FAU - Nakano, H
AU  - Nakano H
FAU - Miyatake, S
AU  - Miyatake S
FAU - Saito, T
AU  - Saito T
LA  - eng
SI  - GENBANK/AF017633
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CD3 Complex)
RN  - 0 (CD3E protein, human)
RN  - 0 (CD3EAP protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-2)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - *CD3 Complex
MH  - Carrier Proteins/*chemistry/*physiology
MH  - DNA, Complementary/chemistry
MH  - Humans
MH  - Interleukin-2/*biosynthesis
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Jurkat Cells
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Receptors, Antigen, T-Cell/*metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes/*metabolism
MH  - *Transcriptional Activation
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18173 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18173-80. doi: 10.1074/jbc.274.26.18173.