PMID- 10373412
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 26
DP  - 1999 Jun 25
TI  - Agonist-dependent interaction of the rat somatostatin receptor subtype 2 with
      cortactin-binding protein 1.
PG  - 18153-6
AB  - We report here an interaction between the C terminus of the rat somatostatin
      receptor subtype 2 (SSTR2) and a protein that has recently been identified as
      cortactin-binding protein 1 (CortBP1). Interaction is mediated by the PDZ
      (PSD-95/discs large/ZO-1) domain of CortBP1. As shown by in situ hybridization,
      SSTR2 and cortactin-binding protein are coexpressed in the rat brain. The
      association between SSTR2 and the PDZ-domain of CortBP1 was verified by overlay
      assays and by coprecipitation after transfection in human embryonic kidney (HEK) 
      cells. Analysis by confocal microscopy indicates that CortBP1 is distributed
      diffusely throughout the cytosol in transfected cells and that it becomes
      concentrated at the plasma membrane when SSTR2 is present. This process is
      largely increased when the receptor is stimulated by somatostatin; as CortBP1
      interacts with the C terminus of SSTR2, our data suggest that the binding of
      agonist to the receptor increase the accessibility of the receptor C terminus to 
      the PDZ domain of CortBP1. Our data for the first time establish a link between a
      G-protein coupled receptor and constituents of the cytoskeleton.
FAU - Zitzer, H
AU  - Zitzer H
AD  - Institut fur Zellbiochemie und klinische Neurobiologie, Universitat Hamburg,
      Martinistrasse 52, 20246 Hamburg, Germany.
FAU - Richter, D
AU  - Richter D
FAU - Kreienkamp, H J
AU  - Kreienkamp HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Somatostatin)
RN  - 0 (Shank2 protein, rat)
RN  - D73QL0OMU2 (somatostatin receptor 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*metabolism
MH  - Cell Line
MH  - Cytoskeletal Proteins/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*metabolism
MH  - Protein Binding
MH  - Rats
MH  - Receptors, Somatostatin/*metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1074/jbc.274.26.18153 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 25;274(26):18153-6. doi: 10.1074/jbc.274.26.18153.