PMID- 10373309
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20191210
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 211
IP  - 1
DP  - 1999 Jul 1
TI  - Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes 
      to microtubules of manchette and axoneme.
PG  - 109-23
AB  - Outer dense fibers are structures unique to the sperm tail. No definite function 
      for these fibers has been found, but they may play a role in motility and provide
      elastic recoil. Their composition had been described before, but only two of the 
      fiber proteins, Odf1 and Odf2, are cloned. We cloned Odf2 by virtue of its
      functional and specific interaction with Odf1, which, we show, is mediated by a
      leucine zipper. Further work demonstrated that the 84-kDa Odf2 protein localizes 
      to both the cortex and the medulla of the fibers, whereas the 27-kDa Odf1 protein
      is present only in the medulla. Here we report the cloning and characterization
      of a new Odf1-interacting protein, Spag4. Spag4 mRNA is spermatid specific, and
      the 49-kDa Spag4 protein complexes specifically with Odf1, but not Odf2, mediated
      by a leucine zipper. It also self-associates. In contrast to Odf1 and Odf2, Spag4
      protein localizes to two microtubule-containing spermatid structures. Spag4 is
      detectable in the transient manchette and it is associated with the axoneme in
      elongating spermatids and epididymal sperm. Our data suggest a role for Spag4 in 
      protein localization to two major sperm tail structures.
CI  - Copyright 1999 Academic Press.
FAU - Shao, X
AU  - Shao X
AD  - Department of Biochemistry and Molecular Biology, University of Calgary, Calgary,
      Alberta, T2N 4N1, Canada.
FAU - Tarnasky, H A
AU  - Tarnasky HA
FAU - Lee, J P
AU  - Lee JP
FAU - Oko, R
AU  - Oko R
FAU - van der Hoorn, F A
AU  - van der Hoorn FA
LA  - eng
SI  - GENBANK/AF043345
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Carrier Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Odf1 protein, rat)
RN  - 0 (Odf2 protein, rat)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Spag4 protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - *Heat-Shock Proteins
MH  - Immunohistochemistry
MH  - Leucine Zippers/genetics
MH  - Male
MH  - Membrane Proteins
MH  - Microscopy, Immunoelectron
MH  - Microtubules/metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Proteins/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Sperm Tail/*metabolism
MH  - Spermatids/chemistry/ultrastructure
MH  - Testis/*metabolism
MH  - Yeasts/genetics
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1006/dbio.1999.9297 [doi]
AID - S0012-1606(99)99297-7 [pii]
PST - ppublish
SO  - Dev Biol. 1999 Jul 1;211(1):109-23. doi: 10.1006/dbio.1999.9297.