PMID- 10372988
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20190831
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 18
IP  - 4
DP  - 1999 Apr
TI  - Elements regulating the transcription of human interstitial retinoid-binding
      protein (IRBP) gene in cultured retinoblastoma cells.
PG  - 283-91
AB  - PURPOSE: To identify cis-acting elements and trans-acting factors involved in the
      expression of human IRBP gene. METHODS: Transient transfection of WERI-Rb1 and
      HeLa cells, DNase 1 footprinting, gel mobility-shift assay and yeast one-hybrid
      system were used to study the regulatory elements that are involved in the
      expression of human IRBP gene. RESULTS: A region between -1620 and -1411 was
      shown to have enhancer properties. Using nuclear extracts from WERI-Rb1 and HeLa 
      cells, four footprints were identified in the proximal promoter region (-206 to
      +68). The core promoter element IP1 binds to OTX2 in the yeast one-hybrid system.
      By cotransfecting HeLa cells, OTX2 could transactivate the irbp promoter. The
      functions of IP2 (from -119 to -86) and IP3 (from -183 to -147) remain to be
      determined. The region containing the HeLa cell-specific footprint IP4 (from -202
      to -180) could silence the OTX2 transactivation of the irbp promoter. CONCLUSION:
      The 5'-flanking region of irbp contains an enhancer sequence. The possible
      silencer upstream from the core promoter may serve to suppress expression of irbp
      in HeLa cells. When the proximal promoter is used to identify binding proteins in
      a human retina library by the yeast one hybrid system, nine of the identified
      clones contained the cDNA sequence for the homeodomain protein OTX2. Since no
      clones for the homeodomain protein CRX were found, and since OTX2 can
      transcriptionally activate irbp in normally non-expressing HeLa cells, it is
      possible that OTX2 rather than CRX is the transcriptional activator for irbp in
      human photoreceptors.
FAU - Fong, S L
AU  - Fong SL
AD  - Department of Ophthalmology, Indiana University, Indianapolis 46202, USA.
      sfong@iupui.edu
FAU - Fong, W B
AU  - Fong WB
LA  - eng
SI  - GENBANK/AF093138
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OTX2 protein, human)
RN  - 0 (Otx Transcription Factors)
RN  - 0 (Peptide Fragments)
RN  - 0 (Retinol-Binding Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (interstitial retinol-binding protein)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Base Sequence/genetics
MH  - Binding Sites/physiology
MH  - Electrophoresis
MH  - *Eye Proteins
MH  - Gene Expression/physiology
MH  - HeLa Cells
MH  - *Homeodomain Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/metabolism/physiology
MH  - Otx Transcription Factors
MH  - Peptide Fragments/genetics
MH  - Promoter Regions, Genetic/genetics/*physiology
MH  - Retinal Neoplasms/*genetics/pathology
MH  - Retinoblastoma/*genetics/pathology
MH  - Retinol-Binding Proteins/*genetics
MH  - Time Factors
MH  - Trans-Activators/metabolism/physiology
MH  - Transcription, Genetic/physiology
MH  - Transcriptional Activation/physiology
MH  - Tumor Cells, Cultured
EDAT- 1999/06/18 00:00
MHDA- 1999/06/18 00:01
CRDT- 1999/06/18 00:00
PHST- 1999/06/18 00:00 [pubmed]
PHST- 1999/06/18 00:01 [medline]
PHST- 1999/06/18 00:00 [entrez]
AID - 10.1076/ceyr.18.4.283.5360 [doi]
PST - ppublish
SO  - Curr Eye Res. 1999 Apr;18(4):283-91. doi: 10.1076/ceyr.18.4.283.5360.