PMID- 10372971
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20181113
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 15
IP  - 12
DP  - 1998 Dec
TI  - Altered expression of sialylated carbohydrate antigens in HT29 colonic carcinoma 
      cells.
PG  - 1155-63
AB  - To determine whether cell growth conditions impacted carbohydrate expression,
      HT29 cells were gradually transferred from a conventional glucose-containing
      media to a glucose-free galactose containing media. Indirect immunofluorescence
      on acetone fixed cells showed increased expression of sialyl Lewis A antigen
      (CA19-9), sialyl Lewis C (DUPAN2) and Tn/sialyl-Tn on the surface of HT29 cells
      grown in the glucose-free galactose containing media compared to those grown in
      the glucose containing media. Sialyltransferases responsible for the synthesis
      for these sialylated epitopes were Increased in the galactose-fed HT29 cells.
      Media overlying the cells was subjected to isopycnic ultracentrifugation in
      cesium chloride and the fractions derived from both glucose and galactose media
      with equivalent buoyant densities of 1.56 g/L, which are predicted to contain
      mucin glycoforms, were further separated by HPLC using a Mono-Q anion exchange
      column. The chromatograph of eluent from the sample derived from the cells
      growing in the galactose containing media showed an increased peak that reacted
      with the anti-sialyl Lewis A antibody, CA19-9. These results show that alteration
      of in vitro culture conditions may cause HT29 colonic carcinoma cells to alter
      the expression of sialylated carbohydrates.
FAU - Mack, D R
AU  - Mack DR
AD  - Department of Pediatrics, University of Nebraska Medical Center, Omaha 69198,
      USA. drmack@unmc.edu
FAU - Cheng, P W
AU  - Cheng PW
FAU - Perini, F
AU  - Perini F
FAU - Wei, S
AU  - Wei S
FAU - Hollingsworth, M A
AU  - Hollingsworth MA
LA  - eng
GR  - CA57362/CA/NCI NIH HHS/United States
GR  - DK02205/DK/NIDDK NIH HHS/United States
GR  - HL48282/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CA-19-9 Antigen)
RN  - 0 (DU-PAN-2 antigen, human)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - IY9XDZ35W2 (Glucose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Adenocarcinoma/*immunology/pathology
MH  - Antigens, Neoplasm/*immunology/isolation & purification
MH  - Blotting, Western
MH  - CA-19-9 Antigen/*immunology/isolation & purification
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Ion Exchange
MH  - Colonic Neoplasms/*immunology/pathology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Galactose
MH  - Glucose
MH  - HT29 Cells
MH  - Humans
MH  - Sialyltransferases/metabolism
EDAT- 1999/06/18 00:00
MHDA- 1999/06/18 00:01
CRDT- 1999/06/18 00:00
PHST- 1999/06/18 00:00 [pubmed]
PHST- 1999/06/18 00:01 [medline]
PHST- 1999/06/18 00:00 [entrez]
PST - ppublish
SO  - Glycoconj J. 1998 Dec;15(12):1155-63.