PMID- 10372966
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20191024
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 15
IP  - 12
DP  - 1998 Dec
TI  - A novel second isoenzyme of the human
      UDP-N-acetylglucosamine:alpha1,3-D-mannoside
      beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and
      chromosomal assignment.
PG  - 1115-23
AB  - We isolated a novel cDNA encoding a second isoenzyme of
      UDP-N-acetylglucosamine:alpha1,3-D-mannoside
      beta1,4-N-acetylglucosaminyltransferase (GnT-IV; EC 2.4.1.145). The nucleotide
      and deduced amino acid sequences of the cDNA were homologous to those of the
      previously cloned human GnT-IV cDNA (63% and 62% identity, respectively). The new
      cDNA is also confirmed to express GnT-IV activity, suggesting that two isoenzymes
      of human GnT-IV exist. Although genomic Southern analysis suggested that both
      genes exist in many mammalian species and the chicken, northern analysis revealed
      that both genes are expressed in different ways in human tissues. This is the
      first report concerning the gene family of an N-acetylglucosaminyltransferase in 
      mammals.
FAU - Yoshida, A
AU  - Yoshida A
AD  - Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama,
      Japan.
FAU - Minowa, M T
AU  - Minowa MT
FAU - Takamatsu, S
AU  - Takamatsu S
FAU - Hara, T
AU  - Hara T
FAU - Ikenaga, H
AU  - Ikenaga H
FAU - Takeuchi, M
AU  - Takeuchi M
LA  - eng
SI  - GENBANK/AB000624
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.145 (alpha-1,3-mannosylglycoprotein
      beta-1,4-N-acetylglucosaminyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - COS Cells
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 5
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary
MH  - Humans
MH  - Isoenzymes/*genetics
MH  - Molecular Sequence Data
MH  - N-Acetylglucosaminyltransferases/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1999/06/18 00:00
MHDA- 1999/06/18 00:01
CRDT- 1999/06/18 00:00
PHST- 1999/06/18 00:00 [pubmed]
PHST- 1999/06/18 00:01 [medline]
PHST- 1999/06/18 00:00 [entrez]
AID - 10.1023/a:1006951519522 [doi]
PST - ppublish
SO  - Glycoconj J. 1998 Dec;15(12):1115-23. doi: 10.1023/a:1006951519522.