PMID- 10371504
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20131121
IS  - 1078-8956 (Print)
IS  - 1078-8956 (Linking)
VI  - 5
IP  - 6
DP  - 1999 Jun
TI  - The intrinsic factor-vitamin B12 receptor, cubilin, is a high-affinity
      apolipoprotein A-I receptor facilitating endocytosis of high-density lipoprotein.
PG  - 656-61
AB  - Cubilin is the intestinal receptor for the endocytosis of intrinsic
      factor-vitamin B12. However, several lines of evidence, including a high
      expression in kidney and yolk sac, indicate it may have additional functions. We 
      isolated apolipoprotein A-I (apoA-I), the main protein of high-density
      lipoprotein (HDL), using cubilin affinity chromatography. Surface plasmon
      resonance analysis demonstrated a high-affinity binding of apoA-I and HDL to
      cubilin, and cubilin-expressing yolk sac cells showed efficient 125I-HDL
      endocytosis that could be inhibited by IgG antibodies against apoA-I and cubilin.
      The physiological relevance of the cubilin-apoA-I interaction was further
      emphasized by urinary apoA-I loss in some known cases of functional cubilin
      deficiency. Therefore, cubilin is a receptor in epithelial apoA-I/HDL metabolism.
FAU - Kozyraki, R
AU  - Kozyraki R
AD  - Department of Medical Biochemistry, University of Aarhus, Denmark.
FAU - Fyfe, J
AU  - Fyfe J
FAU - Kristiansen, M
AU  - Kristiansen M
FAU - Gerdes, C
AU  - Gerdes C
FAU - Jacobsen, C
AU  - Jacobsen C
FAU - Cui, S
AU  - Cui S
FAU - Christensen, E I
AU  - Christensen EI
FAU - Aminoff, M
AU  - Aminoff M
FAU - de la Chapelle, A
AU  - de la Chapelle A
FAU - Krahe, R
AU  - Krahe R
FAU - Verroust, P J
AU  - Verroust PJ
FAU - Moestrup, S K
AU  - Moestrup SK
LA  - eng
GR  - DK45341/DK/NIDDK NIH HHS/United States
GR  - P30 CA16058/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - 0 (Antibodies)
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Leupeptins)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (intrinsic factor-cobalamin receptor)
RN  - 886U3H6UFF (Chloroquine)
RN  - J97339NR3V (leupeptin)
SB  - IM
MH  - Anemia, Megaloblastic/genetics/metabolism
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Apolipoprotein A-I/*genetics/immunology/*metabolism
MH  - Case-Control Studies
MH  - Chloroquine/pharmacology
MH  - Chromatography, Affinity
MH  - Dog Diseases/genetics/metabolism
MH  - Dogs
MH  - Endocytosis/*physiology
MH  - Epithelial Cells/drug effects/metabolism
MH  - Female
MH  - Humans
MH  - Iodine Radioisotopes/metabolism
MH  - Kidney/metabolism
MH  - Leupeptins/pharmacology
MH  - Lipoproteins, HDL/*metabolism
MH  - Malabsorption Syndromes/genetics/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cell Surface/drug effects/immunology/*metabolism
MH  - Reference Values
MH  - Syndrome
MH  - Vitamin B 12 Deficiency/genetics/metabolism
MH  - Yolk Sac/cytology/drug effects/metabolism
EDAT- 1999/06/17 10:00
MHDA- 2001/03/23 10:01
CRDT- 1999/06/17 10:00
PHST- 1999/06/17 10:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/06/17 10:00 [entrez]
AID - 10.1038/9504 [doi]
PST - ppublish
SO  - Nat Med. 1999 Jun;5(6):656-61. doi: 10.1038/9504.