PMID- 10371034
OWN - NLM
STAT- MEDLINE
DCOM- 19990720
LR  - 20181130
IS  - 0168-6445 (Print)
IS  - 0168-6445 (Linking)
VI  - 23
IP  - 3
DP  - 1999 Jun
TI  - 3'-End processing of pre-mRNA in eukaryotes.
PG  - 277-95
AB  - 3'-Ends of almost all eukaryotic mRNAs are generated by endonucleolytic cleavage 
      and addition of a poly(A) tail. In mammalian cells, the reaction depends on the
      sequence AAUAAA upstream of the cleavage site, a degenerate GU-rich sequence
      element downstream of the cleavage site and stimulatory sequences upstream of
      AAUAAA. Six factors have been identified that carry out the two reactions. With a
      single exception, they have been purified to homogeneity and cDNAs for 11
      subunits have been cloned. Some of the cooperative RNA-protein and
      protein-protein interactions within the processing complex have been analyzed,
      but many details, including the identity of the endonuclease, remain unknown.
      Several examples of regulated polyadenylation are being analyzed at the molecular
      level. In the yeast Saccharomyces cerevisiae, sequences directing cleavage and
      polyadenylation are more degenerate than in metazoans, and a downstream element
      has not been identified. The list of processing factors may be complete now with 
      approximately a dozen polypeptides, but their functions in the reaction are
      largely unknown. 3'-Processing is known to be coupled to transcription. This
      connection is thought to involve interactions of processing factors with the mRNA
      cap as well as with RNA polymerase II.
FAU - Wahle, E
AU  - Wahle E
AD  - Institut fur Biochemie, Martin-Luther-Universitat Halle-Wittenberg, Germany.
      ewahle@biochemtech.uni-halle.de
FAU - Ruegsegger, U
AU  - Ruegsegger U
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - FEMS Microbiol Rev
JT  - FEMS microbiology reviews
JID - 8902526
RN  - 0 (3' Untranslated Regions)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - 3' Untranslated Regions/*genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Molecular Sequence Data
MH  - Poly A/metabolism
MH  - RNA Precursors/*genetics/*metabolism
MH  - *RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/genetics/*metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - Transcription, Genetic
RF  - 141
EDAT- 1999/06/17 00:00
MHDA- 1999/06/17 00:01
CRDT- 1999/06/17 00:00
PHST- 1999/06/17 00:00 [pubmed]
PHST- 1999/06/17 00:01 [medline]
PHST- 1999/06/17 00:00 [entrez]
AID - S0168-6445(99)00008-X [pii]
AID - 10.1111/j.1574-6976.1999.tb00400.x [doi]
PST - ppublish
SO  - FEMS Microbiol Rev. 1999 Jun;23(3):277-95. doi:
      10.1111/j.1574-6976.1999.tb00400.x.