PMID- 10370136
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 296
IP  - 3
DP  - 1999 Jun
TI  - Tissue expression of the vesicle protein pantophysin.
PG  - 499-510
AB  - The cell-type restricted expression of cytoplasmic microvesicle membrane protein 
      isoforms may be a consequence of the functional adaptation of these vesicles to
      the execution of specialized processes in cells of different specialization. To
      characterize the expression of the vesicle protein pantophysin, an isoform of the
      synaptic vesicle proteins synaptophysin and synaptoporin, we have prepared and
      characterized antibodies useful for the immunological detection of pantophysin in
      vitro and in situ. Using these reagents, we show by immunoblot analyses that
      pantophysin expression is not homogeneous but differs significantly between
      various bovine tissues. Furthermore, these differences are not exactly paralleled
      by the expression of other vesicle proteins of the SCAMP (secretory
      carrier-associated membrane protein) and VAMP (vesicle-associated membrane
      protein) types that have previously been localized to pantophysin vesicles in
      cultured cells. By immunofluorescence microscopy, pantophysin expression is seen 
      predominantly in non-neuroendocrine cells with pronounced membrane traffic.
      Pantophysin staining codistributes with SCAMP and VAMP immunoreactivities in most
      instances but differs in some. Remarkably, pantophysin staining in liver is
      restricted to cells surrounding sinusoids and is not detectable in hepatocytes,
      similar to that of the SCAMP and VAMP isoforms as detected by our reagents in
      tissue sections.
FAU - Windoffer, R
AU  - Windoffer R
AD  - Department of Anatomy, Johannes Gutenberg-University Mainz, Becherweg 13, D-55099
      Mainz, Germany.
FAU - Borchert-Stuhltrager, M
AU  - Borchert-Stuhltrager M
FAU - Haass, N K
AU  - Haass NK
FAU - Thomas, S
AU  - Thomas S
FAU - Hergt, M
AU  - Hergt M
FAU - Bulitta, C J
AU  - Bulitta CJ
FAU - Leube, R E
AU  - Leube RE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (R-SNARE Proteins)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/biosynthesis
MH  - Cattle
MH  - Cytoplasmic Granules/*metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Membrane Glycoproteins/*biosynthesis
MH  - Membrane Proteins/biosynthesis
MH  - Organ Specificity
MH  - Protein Isoforms/biosynthesis
MH  - R-SNARE Proteins
EDAT- 1999/06/17 00:00
MHDA- 1999/06/17 00:01
CRDT- 1999/06/17 00:00
PHST- 1999/06/17 00:00 [pubmed]
PHST- 1999/06/17 00:01 [medline]
PHST- 1999/06/17 00:00 [entrez]
AID - 92960499.441 [pii]
AID - 10.1007/s004410051310 [doi]
PST - ppublish
SO  - Cell Tissue Res. 1999 Jun;296(3):499-510. doi: 10.1007/s004410051310.