PMID- 10369927
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 49
IP  - 7-8
DP  - 1999 Jul
TI  - Expression analysis of new Ly49 genes: most transcripts of Ly49j lack the
      transmembrane domain.
PG  - 685-91
AB  - Five new Ly49 genes, named Ly49j-n, have recently been identified in C57BL/6
      mice. This study examined the expression of three of these new genes, Ly49j, k,
      and n. To determine whether the Ly49j, k, and n genes were transcribed,
      gene-specific primers were used to amplify cDNA clones for each gene from C57BL/6
      interleukin-2-activated natural killer (NK) cell cDNA. A full-length cDNA for
      Ly49j was detected which encodes a 267 amino acid protein and shares
      approximately 96% nucleotide identity with Ly49c and i. COS cells transfected
      with the Ly49j cDNA were shown to react with the monoclonal antibody 8H7,
      suggesting that the gene likely encodes a functional protein. Many different
      sized Ly49k and n transcripts were observed, although it is likely that they do
      not encode functional proteins due to missing exons or severe truncations in the 
      open reading frames. Interestingly, the most abundant Ly49j transcript detected
      was shown to lack exon 3, which encodes the transmembrane domain. Similar studies
      performed on the same source of NK cell cDNA using Ly49c- and i-specific primers 
      revealed the presence of transmembrane-less Ly49i transcripts, although at a much
      lower frequency than observed for Ly49j. We also detected Ly49g and h transcripts
      lacking the transmembrane domain. Despite the absence of the transmembrane
      region, the resulting Ly49 transcripts maintain their open reading frames, and
      therefore could potentially encode cytoplasmic proteins with a role in NK cell
      function.
FAU - McQueen, K L
AU  - McQueen KL
AD  - Terry Fox Laboratory, B.C. Cancer Agency and the Department of Medical Genetics, 
      University of British Columbia, 601 W10th Avenue, Vancouver, B.C. V5Z 1L3,
      Canada.
FAU - Lohwasser, S
AU  - Lohwasser S
FAU - Takei, F
AU  - Takei F
FAU - Mager, D L
AU  - Mager DL
LA  - eng
SI  - GENBANK/AF110492
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Antigens, Ly)
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA, Complementary)
RN  - 0 (Klra10 protein, mouse)
RN  - 0 (Klra3 protein, mouse)
RN  - 0 (Klra7 protein, mouse)
RN  - 0 (Lectins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily A)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, NK Cell Lectin-Like)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, Ly
MH  - Antigens, Surface/*genetics
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Membrane/metabolism
MH  - DNA, Complementary
MH  - Gene Expression
MH  - Lectins/genetics
MH  - Lectins, C-Type
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - NK Cell Lectin-Like Receptor Subfamily A
MH  - Protein Isoforms
MH  - RNA, Messenger
MH  - Receptors, Immunologic/*genetics
MH  - Receptors, NK Cell Lectin-Like
EDAT- 1999/06/17 00:00
MHDA- 1999/06/17 00:01
CRDT- 1999/06/17 00:00
PHST- 1999/06/17 00:00 [pubmed]
PHST- 1999/06/17 00:01 [medline]
PHST- 1999/06/17 00:00 [entrez]
AID - 90490685.251 [pii]
AID - 10.1007/s002510050665 [doi]
PST - ppublish
SO  - Immunogenetics. 1999 Jul;49(7-8):685-91. doi: 10.1007/s002510050665.