PMID- 10369681
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 12
DP  - 1999 Jun 15
TI  - Aiolos transcription factor controls cell death in T cells by regulating Bcl-2
      expression and its cellular localization.
PG  - 3419-30
AB  - We searched for proteins that interact with Ras in interleukin (IL)-2-stimulated 
      or IL-2-deprived cells, and found that the transcription factor Aiolos interacts 
      with Ras. The Ras-Aiolos interaction was confirmed in vitro and in vivo by
      co-immunoprecipitation. Indirect immunofluorescence shows that IL-2 controls the 
      cellular distribution of Aiolos and induces its tyrosine phosphorylation,
      required for dissociation from Ras. We also identified functional Aiolos-binding 
      sites in the Bcl-2 promoter, which are able to activate the luciferase reporter
      gene. Mutation of Aiolos-binding sites within the Bcl-2 promoter inhibits
      transactivation of the reporter gene luciferase, suggesting direct control of
      Bcl-2 expression by Aiolos. Co-transfection experiments confirm that Aiolos
      induces Bcl-2 expression and prevents apoptosis in IL-2-deprived cells. We
      propose a model for the regulation of Bcl-2 expression via Aiolos.
FAU - Romero, F
AU  - Romero F
AD  - Laboratoire d'Oncologie Cellulaire et Moleculaire, INSERM U363, Hopital Cochin,
      27 rue du Faubourg Saint-Jacques, F-75014 Paris, France.
FAU - Martinez-A, C
AU  - Martinez-A C
FAU - Camonis, J
AU  - Camonis J
FAU - Rebollo, A
AU  - Rebollo A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (IKZF3 protein, human)
RN  - 0 (Ikzf3 protein, mouse)
RN  - 0 (Interleukin-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 148971-36-2 (Ikaros Transcription Factor)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 3.6.5.2 (HRAS protein, human)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cytoplasm/drug effects/metabolism
MH  - Guanosine Diphosphate/metabolism
MH  - Humans
MH  - Ikaros Transcription Factor
MH  - Interleukin-2/pharmacology/physiology
MH  - Mice
MH  - Mutation
MH  - Phosphotyrosine/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics/metabolism
MH  - Proto-Oncogene Proteins p21(ras)/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/metabolism
MH  - T-Lymphocytes/*cytology/drug effects/metabolism
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors
MH  - *Transcriptional Activation/drug effects
MH  - Yeasts/genetics
PMC - PMC1171421
EDAT- 1999/06/16 00:00
MHDA- 1999/06/16 00:01
CRDT- 1999/06/16 00:00
PHST- 1999/06/16 00:00 [pubmed]
PHST- 1999/06/16 00:01 [medline]
PHST- 1999/06/16 00:00 [entrez]
AID - 10.1093/emboj/18.12.3419 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jun 15;18(12):3419-30. doi: 10.1093/emboj/18.12.3419.