PMID- 10369680
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 12
DP  - 1999 Jun 15
TI  - RYBP, a new repressor protein that interacts with components of the mammalian
      Polycomb complex, and with the transcription factor YY1.
PG  - 3404-18
AB  - The products of the Polycomb group (PcG) of genes are necessary for the
      maintenance of transcriptional repression of a number of important developmental 
      genes, including the homeotic genes. A two-hybrid screen was used to search for
      putative new members of the PcG of genes in mammals. We have identified a new Zn 
      finger protein, RYBP, which interacts directly with both Ring1 proteins (Ring1A
      and Ring1B) and with M33, two mutually interacting sets of proteins of the
      mammalian Polycomb complex. Ring1 binds RYBP and M33 through the same C-terminal 
      domain, whereas the RYBP-M33 interaction takes place through an M33 domain not
      involved in Ring1 binding. RYBP also interacts directly with YY1, a transcription
      factor partially related to the product of the Drosophila pleiohomeotic gene. In 
      addition, we show here that RYBP acts as a transcriptional repressor in
      transiently transfected cells. Finally, RYBP shows a dynamic expression pattern
      during embryogenesis which initially overlaps partially that of Ring1A in the
      central nervous system, and later becomes ubiquitous. Taken together, these data 
      suggest that RYBP may play a relevant role in PcG function in mammals.
FAU - Garcia, E
AU  - Garcia E
AD  - Centro de Investigaciones Biologicas, Department of Developmental and Cell
      Biology, Velazquez 144, 28006 Madrid, Spain.
FAU - Marcos-Gutierrez, C
AU  - Marcos-Gutierrez C
FAU - del Mar Lorente, M
AU  - del Mar Lorente M
FAU - Moreno, J C
AU  - Moreno JC
FAU - Vidal, M
AU  - Vidal M
LA  - eng
SI  - GENBANK/AF101779
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Cbx2 protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (Polycomb-Group Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Rybp protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (YY1 Transcription Factor)
RN  - 0 (YY1 protein, human)
RN  - 0 (Yy1 protein, mouse)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
RN  - EC 2.3.2.27 (RING1 protein, human)
RN  - EC 2.3.2.27 (RNF2 protein, human)
RN  - EC 2.3.2.27 (Ring1 protein, mouse)
RN  - EC 2.3.2.27 (Rnf2 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Central Nervous System/embryology/metabolism
MH  - Cloning, Molecular
MH  - Cytoplasm/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Embryo, Mammalian/metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polycomb Repressive Complex 1
MH  - Polycomb-Group Proteins
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Repressor Proteins/chemistry/genetics/*metabolism
MH  - Sequence Deletion
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcription, Genetic/genetics
MH  - Ubiquitin-Protein Ligases
MH  - YY1 Transcription Factor
MH  - Yeasts/genetics
MH  - Zinc Fingers
PMC - PMC1171420
EDAT- 1999/06/16 00:00
MHDA- 1999/06/16 00:01
CRDT- 1999/06/16 00:00
PHST- 1999/06/16 00:00 [pubmed]
PHST- 1999/06/16 00:01 [medline]
PHST- 1999/06/16 00:00 [entrez]
AID - 10.1093/emboj/18.12.3404 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jun 15;18(12):3404-18. doi: 10.1093/emboj/18.12.3404.