PMID- 10369678
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20190524
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 12
DP  - 1999 Jun 15
TI  - A mosaic analysis in Drosophila fat body cells of the control of antimicrobial
      peptide genes by the Rel proteins Dorsal and DIF.
PG  - 3380-91
AB  - Expression of the gene encoding the antifungal peptide Drosomycin in Drosophila
      adults is controlled by the Toll signaling pathway. The Rel proteins Dorsal and
      DIF (Dorsal-related immunity factor) are possible candidates for the
      transactivating protein in the Toll pathway that directly regulates the
      drosomycin gene. We have examined the requirement of Dorsal and DIF for
      drosomycin expression in larval fat body cells, the predominant immune-responsive
      tissue, using the yeast site-specific flp/FRT recombination system to generate
      cell clones homozygous for a deficiency uncovering both the dorsal and the dif
      genes. Here we show that in the absence of both genes, the immune-inducibility of
      drosomycin is lost but can be rescued by overexpression of either dorsal or dif
      under the control of a heat-shock promoter. This result suggests a functional
      redundancy between both Rel proteins in the control of drosomycin gene expression
      in the larvae of Drosophila. Interestingly, the gene encoding the antibacterial
      peptide Diptericin remains fully inducible in the absence of the dorsal and dif
      genes. Finally, we have used fat body cell clones homozygous for various
      mutations to show that a linear activation cascade Spaetzle-->
      Toll-->Cactus-->Dorsal/DIF leads to the induction of the drosomycin gene in
      larval fat body cells.
FAU - Manfruelli, P
AU  - Manfruelli P
AD  - Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du Centre National de la
      Recherche Scientifique, 15, Rue Rene Descartes, F-67084 Strasbourg Cedex, France.
FAU - Reichhart, J M
AU  - Reichhart JM
FAU - Steward, R
AU  - Steward R
FAU - Hoffmann, J A
AU  - Hoffmann JA
FAU - Lemaitre, B
AU  - Lemaitre B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Anti-Infective Agents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dif protein, Drosophila)
RN  - 0 (DptA protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tl protein, Drosophila)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factors)
RN  - 0 (dl protein, Drosophila)
RN  - 0 (spz protein, Drosophila)
RN  - 149059-01-8 (cact protein, Drosophila)
RN  - 159522-55-1 (DRS protein, Drosophila)
SB  - IM
MH  - Animals
MH  - *Anti-Infective Agents
MH  - Clone Cells/metabolism
MH  - DNA-Binding Proteins/genetics/*physiology
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/cytology/*genetics/immunology
MH  - Fat Body/*metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Genes, Insect
MH  - Genes, Reporter
MH  - Insect Proteins/*genetics/physiology
MH  - Larva/cytology/genetics
MH  - Male
MH  - Membrane Glycoproteins/genetics/physiology
MH  - Mosaicism
MH  - Mutation
MH  - Nuclear Proteins/genetics/*physiology
MH  - Phosphoproteins/genetics/*physiology
MH  - *Receptors, Cell Surface
MH  - Signal Transduction/genetics/physiology
MH  - Toll-Like Receptors
MH  - *Transcription Factors
PMC - PMC1171418
EDAT- 1999/06/16 00:00
MHDA- 1999/06/16 00:01
CRDT- 1999/06/16 00:00
PHST- 1999/06/16 00:00 [pubmed]
PHST- 1999/06/16 00:01 [medline]
PHST- 1999/06/16 00:00 [entrez]
AID - 10.1093/emboj/18.12.3380 [doi]
PST - ppublish
SO  - EMBO J. 1999 Jun 15;18(12):3380-91. doi: 10.1093/emboj/18.12.3380.